TY - JOUR
T1 - The Genome of Anopheles darlingi, the main neotropical malaria vector
AU - Marinotti, Osvaldo
AU - Cerqueira, Gustavo C.
AU - De Almeida, Luiz Gonzaga Paula
AU - Ferro, Maria Inês Tiraboschi
AU - Da Silva Loreto, Elgion Lucio
AU - Zaha, Arnaldo
AU - Teixeira, Santuza M.R.
AU - Wespiser, Adam R.
AU - E Silva, Alexandre Almeida
AU - Schlindwein, Aline Daiane
AU - Pacheco, Ana Carolina Landim
AU - Da Costa Da Silva, Artur Luiz
AU - Graveley, Brenton R.
AU - Walenz, Brian P.
AU - De Araujo Lima, Bruna
AU - Ribeiro, Carlos Alexandre Gomes
AU - Nunes-Silva, Carlos Gustavo
AU - De Carvalho, Carlos Roberto
AU - De Almeida Soares, Célia Maria
AU - De Menezes, Claudia Beatriz Afonso
AU - Matiolli, Cleverson
AU - Caffrey, Daniel
AU - Araújo, Demetrius Antonio M.
AU - De Oliveira, Diana Magalhães
AU - Golenbock, Douglas
AU - Grisard, Edmundo Carlos
AU - Fantinatti-Garboggini, Fabiana
AU - De Carvalho, Fabíola Marques
AU - Barcellos, Fernando Gomes
AU - Prosdocimi, Francisco
AU - May, Gemma
AU - De Azevedo Junior, Gilson Martins
AU - Guimarães, Giselle Moura
AU - Goldman, Gustavo Henrique
AU - Padilha, Itácio Q.M.
AU - Da Silva Batista, Jacqueline
AU - Ferro, Jesus Aparecido
AU - Ribeiro, José M.C.
AU - Fietto, Juliana Lopes Rangel
AU - Dabbas, Karina Maia
AU - Cerdeira, Louise
AU - Agnez-Lima, Lucymara Fassarella
AU - Brocchi, Marcelo
AU - De Carvalho, Marcos Oliveira
AU - De Melo Teixeira, Marcus
AU - De Mascena Diniz Maia, Maria
AU - Goldman, Maria Helena S.
AU - Schneider, Maria Paula Cruz
AU - Felipe, Maria Sueli Soares
AU - Hungria, Mariangela
AU - Nicolás, Marisa Fabiana
AU - Pereira, Maristela
AU - Montes, Martín Alejandro
AU - Cantão, Maurício E.
AU - Vincentz, Michel
AU - Rafael, Miriam Silva
AU - Silverman, Neal
AU - Stoco, Patrícia Hermes
AU - Souza, Rangel Celso
AU - Vicentini, Renato
AU - Gazzinelli, Ricardo Tostes
AU - De Oliveira Neves, Rogério
AU - Silva, Rosane
AU - Astolfi-Filho, Spartaco
AU - Maciel, Talles Eduardo Ferreira
AU - Ürményi, Turàn P.
AU - Tadei, Wanderli Pedro
AU - Camargo, Erney Plessmann
AU - De Vasconcelos, Ana Tereza Ribeiro
PY - 2013/8/1
Y1 - 2013/8/1
N2 - Anopheles darlingi is the principal neotropical malaria vector, responsible for more than a million cases of malaria per year on the American continent. Anopheles darlingi diverged from the African and Asian malaria vectors ∼100 million years ago (mya) and successfully adapted to the New World environment. Here we present an annotated reference A. darlingi genome, sequenced from a wild population of males and females collected in the Brazilian Amazon. A total of 10 481 predicted protein-coding genes were annotated, 72% of which have their closest counterpart in Anopheles gambiae and 21% have highest similarity with other mosquito species. In spite of a long period of divergent evolution, conserved gene synteny was observed between A. darlingi and A. gambiae. More than 10 million single nucleotide polymorphisms and short indels with potential use as genetic markers were identified. Transposable elements correspond to 2.3% of the A. darlingi genome. Genes associated with hematophagy, immunity and insecticide resistance, directly involved in vectorhuman and vectorparasite interactions, were identified and discussed. This study represents the first effort to sequence the genome of a neotropical malaria vector, and opens a new window through which we can contemplate the evolutionary history of anopheline mosquitoes. It also provides valuable information that may lead to novel strategies to reduce malaria transmission on the South American continent. The A. darlingi genome is accessible at www.labinfo.lncc.br/index.php/anopheles- darlingi.
AB - Anopheles darlingi is the principal neotropical malaria vector, responsible for more than a million cases of malaria per year on the American continent. Anopheles darlingi diverged from the African and Asian malaria vectors ∼100 million years ago (mya) and successfully adapted to the New World environment. Here we present an annotated reference A. darlingi genome, sequenced from a wild population of males and females collected in the Brazilian Amazon. A total of 10 481 predicted protein-coding genes were annotated, 72% of which have their closest counterpart in Anopheles gambiae and 21% have highest similarity with other mosquito species. In spite of a long period of divergent evolution, conserved gene synteny was observed between A. darlingi and A. gambiae. More than 10 million single nucleotide polymorphisms and short indels with potential use as genetic markers were identified. Transposable elements correspond to 2.3% of the A. darlingi genome. Genes associated with hematophagy, immunity and insecticide resistance, directly involved in vectorhuman and vectorparasite interactions, were identified and discussed. This study represents the first effort to sequence the genome of a neotropical malaria vector, and opens a new window through which we can contemplate the evolutionary history of anopheline mosquitoes. It also provides valuable information that may lead to novel strategies to reduce malaria transmission on the South American continent. The A. darlingi genome is accessible at www.labinfo.lncc.br/index.php/anopheles- darlingi.
UR - http://www.scopus.com/inward/record.url?scp=84883471432&partnerID=8YFLogxK
U2 - 10.1093/nar/gkt484
DO - 10.1093/nar/gkt484
M3 - Article
C2 - 23761445
AN - SCOPUS:84883471432
SN - 0305-1048
VL - 41
SP - 7387
EP - 7400
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 15
ER -