The genes encoding mammalian chaperonin 60 and chaperonin 10 are linked head-to-head and share a bidirectional promoter

Michael T. Ryan, Susanna M. Herd, Gian Sberna, Melanie M. Samuel, Nicholas J. Hoogenraad, Peter B. Høj

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39 Citations (Scopus)

Abstract

Chaperonins are a class of stress-inducible molecular chaperones involved in protein folding. We report the cloning, sequencing and characterisation of the rat mitochondrial chaperonin 60 and chaperonin 10 genes. The two genes are arranged in a head-to-head configuration and together comprise 14 kb and contain 14 introns. The genes are linked together by a region of approximately 280 bp, which constitutes a bidirectional promoter and includes a common heat-shock element. Insertion of the shared promoter region between two reporter genes is sufficient to drive their expression under both constitutive and heat-shock conditions. The arrangement of the mammalian chaperonin genes suggests the potential to provide the coordinated regulation of their products in a manner that is mechanistically distinct from, yet conceptually similar to, that employed by the bacterial chaperonin (groE) operon.

Original languageEnglish
Pages (from-to)9-17
Number of pages9
JournalGene
Volume196
Issue number1-2
DOIs
Publication statusPublished - 1 Sep 1997
Externally publishedYes

Keywords

  • Heat shock
  • Hsp10
  • Hsp60
  • Stress protein

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