The genes encoding cAMP-dependent protein kinase catalytic subunit homologues of the microsporidia Encephalitozoon intestinalis and E. cuniculi: Molecular characterisation and phylogenetic analysis

Leila Equinet, Eric Bapteste, Marc Thellier, Meryem Ouarzane-Amara, Christian P. Vivarès, Isabelle Desportes-Livage, Christian Doerig

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3 Citations (Scopus)

Abstract

A gene encoding a protein kinase was identified by homology-based PCR amplification in Encephalitozoon intestinalis, a microsporidian parasite pathogenic to humans, and its orthologue has been identified by database mining in the genome of the related species E. cuniculi, whose sequence has been recently published. Phylogenetic analysis revealed that the proteins encoded by these genes are homologues of the cAMP-dependent protein kinase catalytic subunits (PKAc). Southern blot analysis indicated that the EiPKAc gene is present in two copies in the E. intestinalis genome, whereas the E. cuniculi orthologue (EcPKAc) is a single copy gene. RT-PCR data showed that the EiPKAc gene is expressed in at least one of the intracellular stages during infection of the mammalian host cell by E. intestinalis.

Original languageEnglish
Pages (from-to)277-285
Number of pages9
JournalParasitology International
Volume53
Issue number4
DOIs
Publication statusPublished - 1 Dec 2004
Externally publishedYes

Keywords

  • bp, base pair
  • cAMP, cyclic adenosine monophosphate
  • EST, expressed sequence tag
  • kb, kilobase
  • kDa, kilodalton
  • Microsporidia
  • ORF, open reading frame
  • PCR, polymerase chain reaction
  • Phosphorylation
  • Phylogeny
  • Protein kinase
  • Protein kinase A

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