TY - JOUR
T1 - The first isolation and whole genome sequencing of murray valley encephalitis virus from cerebrospinal fluid of a patient with encephalitis
AU - Russell, Jessica S.
AU - Caly, Leon
AU - Kostecki, Renata
AU - McGuinness, Sarah L.
AU - Carter, Glen
AU - Bulach, Dieter
AU - Seemann, Torsten
AU - Stinear, Tim P.
AU - Baird, Rob
AU - Catton, Mike
AU - Druce, Julian
PY - 2018/6/11
Y1 - 2018/6/11
N2 - Murray Valley Encephalitis virus (MVEV) is amosquito-borne Flavivirus. Clinical presentation is rare but severe, with a case fatality rate of 15–30%. Here we report a case of MVEV from the cerebrospinal fluid (CSF) of a patient in the Northern Territory in Australia. Initial diagnosis was performed using both MVEV-specific real-time, and Pan-Flavivirus conventional, Polymerase Chain Reaction (PCR), with confirmation by Sanger sequencing. Subsequent isolation, the first from CSF, was conducted in Vero cells and the observed cytopathic effect was confirmed by increasing viral titre in the real-time PCR. Isolation allowed for full genome sequencing using the Scriptseq V2 RNASeq library preparation kit. A consensus genome for VIDRL-MVE was generated and phylogenetic analysis identified it as Genotype 2. This is the first reported isolation, and full genome sequencing of MVEV from CSF. It is also the first time Genotype 2 has been identified in humans. As such, this case has significant implications for public health surveillance, epidemiology, and the understanding of MVEV evolution.
AB - Murray Valley Encephalitis virus (MVEV) is amosquito-borne Flavivirus. Clinical presentation is rare but severe, with a case fatality rate of 15–30%. Here we report a case of MVEV from the cerebrospinal fluid (CSF) of a patient in the Northern Territory in Australia. Initial diagnosis was performed using both MVEV-specific real-time, and Pan-Flavivirus conventional, Polymerase Chain Reaction (PCR), with confirmation by Sanger sequencing. Subsequent isolation, the first from CSF, was conducted in Vero cells and the observed cytopathic effect was confirmed by increasing viral titre in the real-time PCR. Isolation allowed for full genome sequencing using the Scriptseq V2 RNASeq library preparation kit. A consensus genome for VIDRL-MVE was generated and phylogenetic analysis identified it as Genotype 2. This is the first reported isolation, and full genome sequencing of MVEV from CSF. It is also the first time Genotype 2 has been identified in humans. As such, this case has significant implications for public health surveillance, epidemiology, and the understanding of MVEV evolution.
KW - Cerebrospinal fluid
KW - Flavivirus
KW - Isolation
KW - Murray Valley encephalitis
KW - Whole genome sequencing
UR - http://www.scopus.com/inward/record.url?scp=85048719268&partnerID=8YFLogxK
U2 - 10.3390/v10060319
DO - 10.3390/v10060319
M3 - Article
AN - SCOPUS:85048719268
SN - 1999-4915
VL - 10
JO - Viruses
JF - Viruses
IS - 6
M1 - 319
ER -