The extracellular matrix regulates MaeuCath1a gene expression

Stephen Wanyonyi, Christophe Lefevre, Julie A. Sharp, Kevin R. Nicholas

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

We have previously shown that the gene for MaeuCath1, a cathelicidin secreted in wallaby milk is alternately spliced into two variants, MaeuCath1a and MaeuCath1b which are temporally regulated in order to provide antimicrobial protection to the newborn and stimulate mammary growth, respectively. The current study investigated the extracellular matrix (ECM) for its regulatory role in MaeuCath1 gene expression. Reverse transcription qPCR using RNA isolated from mammary epithelial cells (WallMEC) cultured on ECM showed that ECM regulates MaeuCath1a gene expression in a lactation phase-dependent manner. Luciferase reporter-based assays and in silico analysis of deletion fragments of the 2245. bp sequence upstream of the translation start site identified ECM-dependent positive regulatory activity in the -709 to -15 region and repressor activity in the -919 to -710 region. Electrophoretic Gel Mobility Shift Assays (EMSA) using nuclear extract from ECM-treated WallMEC showed differential band shift in the -839 to -710 region.

Original languageEnglish
Pages (from-to)289-299
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume40
Issue number3-4
DOIs
Publication statusPublished - Jul 2013
Externally publishedYes

Keywords

  • Cathelicidin
  • Cathelicidin promoter
  • Extracellular matrix
  • Gene regulation
  • Lactation
  • Mammary epithelial cells

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