The exported chaperone Hsp70-x supports virulence functions for Plasmodium falciparum blood stage parasites

Sarah C. Charnaud, Matthew W A Dixon, Catherine Q Nie, Lia Chappell, Paul R Sanders, Thomas Nebl, Eric Hanssen, Matthew Berriman, Jo-anne Chan, Adam J. Blanch, James G. Beeson, Julian C Rayner, Jude M Przyborski, Leann Tilley, Brendan S. Crabb, Paul R. Gilson

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10 Citations (Scopus)

Abstract

Malaria is caused by five different Plasmodium spp. in humans each of which modifies the host erythrocyte to survive and replicate. The two main causes of malaria, P. falciparum and P. vivax, differ in their ability to cause severe disease, mainly due to differences in the cytoadhesion of infected erythrocytes (IE) in the microvasculature. Cytoadhesion of P. falciparum in the brain leads to a large number of deaths each year and is a consequence of exported parasite proteins, some of which modify the erythrocyte cytoskeleton while others such as PfEMP1 project onto the erythrocyte surface where they bind to endothelial cells. Here we investigate the effects of knocking out an exported Hsp70-type chaperone termed Hsp70-x that is present in P. falciparum but not P. vivax. Although the growth of Δhsp70-x parasites was unaffected, the export of PfEMP1 cytoadherence proteins was delayed and Δhsp70-x IE had reduced adhesion. The Δhsp70-x IE were also more rigid than wild-type controls indicating changes in the way the parasites modified their host erythrocyte. To investigate the cause of this, transcriptional and translational changes in exported and chaperone proteins were monitored and some changes were observed. We propose that PfHsp70-x is not essential for survival in vitro, but may be required for the efficient export and functioning of some P. falciparum exported proteins.

Original languageEnglish
Article numbere0181656
Number of pages22
JournalPLoS ONE
Volume12
Issue number7
DOIs
Publication statusPublished - 1 Jul 2017

Cite this

Charnaud, S. C., Dixon, M. W. A., Nie, C. Q., Chappell, L., Sanders, P. R., Nebl, T., ... Gilson, P. R. (2017). The exported chaperone Hsp70-x supports virulence functions for Plasmodium falciparum blood stage parasites. PLoS ONE, 12(7), [e0181656]. https://doi.org/10.1371/journal.pone.0181656
Charnaud, Sarah C. ; Dixon, Matthew W A ; Nie, Catherine Q ; Chappell, Lia ; Sanders, Paul R ; Nebl, Thomas ; Hanssen, Eric ; Berriman, Matthew ; Chan, Jo-anne ; Blanch, Adam J. ; Beeson, James G. ; Rayner, Julian C ; Przyborski, Jude M ; Tilley, Leann ; Crabb, Brendan S. ; Gilson, Paul R. / The exported chaperone Hsp70-x supports virulence functions for Plasmodium falciparum blood stage parasites. In: PLoS ONE. 2017 ; Vol. 12, No. 7.
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abstract = "Malaria is caused by five different Plasmodium spp. in humans each of which modifies the host erythrocyte to survive and replicate. The two main causes of malaria, P. falciparum and P. vivax, differ in their ability to cause severe disease, mainly due to differences in the cytoadhesion of infected erythrocytes (IE) in the microvasculature. Cytoadhesion of P. falciparum in the brain leads to a large number of deaths each year and is a consequence of exported parasite proteins, some of which modify the erythrocyte cytoskeleton while others such as PfEMP1 project onto the erythrocyte surface where they bind to endothelial cells. Here we investigate the effects of knocking out an exported Hsp70-type chaperone termed Hsp70-x that is present in P. falciparum but not P. vivax. Although the growth of Δhsp70-x parasites was unaffected, the export of PfEMP1 cytoadherence proteins was delayed and Δhsp70-x IE had reduced adhesion. The Δhsp70-x IE were also more rigid than wild-type controls indicating changes in the way the parasites modified their host erythrocyte. To investigate the cause of this, transcriptional and translational changes in exported and chaperone proteins were monitored and some changes were observed. We propose that PfHsp70-x is not essential for survival in vitro, but may be required for the efficient export and functioning of some P. falciparum exported proteins.",
author = "Charnaud, {Sarah C.} and Dixon, {Matthew W A} and Nie, {Catherine Q} and Lia Chappell and Sanders, {Paul R} and Thomas Nebl and Eric Hanssen and Matthew Berriman and Jo-anne Chan and Blanch, {Adam J.} and Beeson, {James G.} and Rayner, {Julian C} and Przyborski, {Jude M} and Leann Tilley and Crabb, {Brendan S.} and Gilson, {Paul R.}",
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Charnaud, SC, Dixon, MWA, Nie, CQ, Chappell, L, Sanders, PR, Nebl, T, Hanssen, E, Berriman, M, Chan, J, Blanch, AJ, Beeson, JG, Rayner, JC, Przyborski, JM, Tilley, L, Crabb, BS & Gilson, PR 2017, 'The exported chaperone Hsp70-x supports virulence functions for Plasmodium falciparum blood stage parasites', PLoS ONE, vol. 12, no. 7, e0181656. https://doi.org/10.1371/journal.pone.0181656

The exported chaperone Hsp70-x supports virulence functions for Plasmodium falciparum blood stage parasites. / Charnaud, Sarah C.; Dixon, Matthew W A; Nie, Catherine Q; Chappell, Lia; Sanders, Paul R; Nebl, Thomas; Hanssen, Eric; Berriman, Matthew; Chan, Jo-anne; Blanch, Adam J.; Beeson, James G.; Rayner, Julian C; Przyborski, Jude M; Tilley, Leann; Crabb, Brendan S.; Gilson, Paul R.

In: PLoS ONE, Vol. 12, No. 7, e0181656, 01.07.2017.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - The exported chaperone Hsp70-x supports virulence functions for Plasmodium falciparum blood stage parasites

AU - Charnaud, Sarah C.

AU - Dixon, Matthew W A

AU - Nie, Catherine Q

AU - Chappell, Lia

AU - Sanders, Paul R

AU - Nebl, Thomas

AU - Hanssen, Eric

AU - Berriman, Matthew

AU - Chan, Jo-anne

AU - Blanch, Adam J.

AU - Beeson, James G.

AU - Rayner, Julian C

AU - Przyborski, Jude M

AU - Tilley, Leann

AU - Crabb, Brendan S.

AU - Gilson, Paul R.

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AB - Malaria is caused by five different Plasmodium spp. in humans each of which modifies the host erythrocyte to survive and replicate. The two main causes of malaria, P. falciparum and P. vivax, differ in their ability to cause severe disease, mainly due to differences in the cytoadhesion of infected erythrocytes (IE) in the microvasculature. Cytoadhesion of P. falciparum in the brain leads to a large number of deaths each year and is a consequence of exported parasite proteins, some of which modify the erythrocyte cytoskeleton while others such as PfEMP1 project onto the erythrocyte surface where they bind to endothelial cells. Here we investigate the effects of knocking out an exported Hsp70-type chaperone termed Hsp70-x that is present in P. falciparum but not P. vivax. Although the growth of Δhsp70-x parasites was unaffected, the export of PfEMP1 cytoadherence proteins was delayed and Δhsp70-x IE had reduced adhesion. The Δhsp70-x IE were also more rigid than wild-type controls indicating changes in the way the parasites modified their host erythrocyte. To investigate the cause of this, transcriptional and translational changes in exported and chaperone proteins were monitored and some changes were observed. We propose that PfHsp70-x is not essential for survival in vitro, but may be required for the efficient export and functioning of some P. falciparum exported proteins.

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