The evolution of low mutation rates in experimental mutator populations of Saccharomyces cerevisiae

Michael J. McDonald, Yu Ying Hsieh, Yen Hsin Yu, Shang Lin Chang, Jun Yi Leu

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30 Citations (Scopus)

Abstract

Mutation is the source of both beneficial adaptive variation and deleterious genetic load, fueling the opposing selective forces than shape mutation rate evolution. This dichotomy is well illustrated by the evolution of the mutator phenotype, a genome-wide 10- to 100-fold increase in mutation rate. This phenotype has often been observed in clonally expanding populations exposed to novel or frequently changing conditions [1-5]. Although studies of both experimental and natural populations have shed light on the evolutionary forces that lead to the spread of the mutator allele through a population [5-11], significant gaps in our understanding of mutator evolution remain [12]. Here we use an experimental evolution approach to investigate the conditions required for the evolution of a reduction in mutation rate and the mechanisms by which populations tolerate the accumulation of deleterious mutations. We find that after ∼6,700 generations, four out of eight experimental mutator lines had evolved a decreased mutation rate. We provide evidence that the accumulation of deleterious mutations leads to selection for reduced mutation rate clones in populations of mutators. Finally, we test the long-term consequences of the mutator phenotype, finding that mutator lines follow different evolutionary trajectories, some of which lead to drug resistance.
Original languageEnglish
Pages (from-to)1235-1240
Number of pages6
JournalCurrent Biology
Volume22
Issue number13
DOIs
Publication statusPublished - 10 Jul 2012
Externally publishedYes

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