The efficient generation of induced pluripotent stem (iPS) cells from adult mouse adipose tissue-derived and neural stem cells

Pollyanna Agnes Tat, Huseyin Sumer, Karen Jones, Kyle Upton, Paul Verma

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    Abstract

    Ectopic expression of key reprogramming transgenes in somatic cells enables them to adopt the characteristics of pluripotency. Such cells have been termed induced pluripotent stem (iPS) cells and have revolutionized the field of somatic cell reprogramming, as the need for embryonic material is obviated. One of the issues facing both the clinical translation of iPS cell technology and the efficient derivation of iPS cell lines in the research laboratory is choosing the most appropriate somatic cell type for induction. In this study, we demonstrate the direct reprogramming of a defined population of neural stem cells (NSCs) derived from the subventricular zone (SVZ) and adipose tissue-derived cells (ADCs) from adult mice using retroviral transduction of the Yamanaka factors Oct4, Sox2, Klf4, and c-Myc, and compared the results obtained with a mouse embryonic fibroblast (mEF) control. We isolated mEFs, NSCs, and ADCs from transgenic mice, which possess a GFP transgene under control of the Oct4 promoter, and validated GFP expression as an indicator of reprogramming. While transduction efficiencies were not significantly different among the different cell types (mEFs 68.70 +/- 2.62 , ADCs 70.61 +/- 15.4 , NSCs, 68.72 +/- 3 , p = 0.97), the number of GFP-positive colonies and hence the number of reprogramming events was significantly higher for both NSCs (13.50 +/- 4.10 colonies, 0.13 +/- 0.06 ) and ADCs (118.20 +/- 38.28 colonies, 1.14 +/- 0.77 ) when compared with the mEF control (3.17 +/- 0.29 colonies, 0.03 +/- 0.005 ).
    Original languageEnglish
    Pages (from-to)525 - 536
    Number of pages12
    JournalCell Transplantation
    Volume19
    Issue number5
    DOIs
    Publication statusPublished - 2010

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