TY - JOUR
T1 - The effects of targeted deletion of the aromatase enzyme on prostatic contractile responses to noradrenaline in mice
AU - Gray, Katherine Teresa
AU - Short, Jennifer Lynn
AU - Simpson, Evan R
AU - Ventura, Sabatino
PY - 2007
Y1 - 2007
N2 - This investigation aimed to see whether a change in the oestrogen to androgen ratio alters prostate contractility. Isolated organ bath studies using prostates from aromatase knockout (ArKO) mice which were homozygous (ArKO-/-) and heterozygous (ArKO+/-) for the disrupted aromatase cyp19 gene and wild-type littermates (ArKO+/+) were conducted. The distribution of noradrenergic nerves was visualized using the sucrose-potassium phosphate-glyoxylic acid method. ArKO-/- mice had increased prostate weights compared with ArKO+/+ mice. Frequency-response curves to electrical field stimulation (EFS; 0.5 ms pulse duration, 60 V, 0.1-20 Hz) yielded frequency-dependent contractions, while noradrenaline (10 nM-1 mM) and tyramine (1 muM-1 mM) produced concentration-dependent contractions. Prazosin (0.3 muM) attenuated the responses induced by noradrenaline and EFS in all mice (P/=0.506, n=10-13). Prostates from ArKO-/- and ArKO+/- mice were more sensitive to tyramine than prostates from ArKO+/+ mice (P
AB - This investigation aimed to see whether a change in the oestrogen to androgen ratio alters prostate contractility. Isolated organ bath studies using prostates from aromatase knockout (ArKO) mice which were homozygous (ArKO-/-) and heterozygous (ArKO+/-) for the disrupted aromatase cyp19 gene and wild-type littermates (ArKO+/+) were conducted. The distribution of noradrenergic nerves was visualized using the sucrose-potassium phosphate-glyoxylic acid method. ArKO-/- mice had increased prostate weights compared with ArKO+/+ mice. Frequency-response curves to electrical field stimulation (EFS; 0.5 ms pulse duration, 60 V, 0.1-20 Hz) yielded frequency-dependent contractions, while noradrenaline (10 nM-1 mM) and tyramine (1 muM-1 mM) produced concentration-dependent contractions. Prazosin (0.3 muM) attenuated the responses induced by noradrenaline and EFS in all mice (P/=0.506, n=10-13). Prostates from ArKO-/- and ArKO+/- mice were more sensitive to tyramine than prostates from ArKO+/+ mice (P
M3 - Article
SN - 0022-0795
VL - 195
SP - 495
EP - 502
JO - Journal of Endocrinology
JF - Journal of Endocrinology
IS - 3
ER -