The effects of relaxin and estrogen deficiency on collagen deposition and hypertrophy of nonreproductive organs

Edna Lekgabe, Simon Royce, Tim Hewitson, Mimi Tang, Chongxin Zhao, Xiao-Lei Moore, Geoffrey Tregear, Ross Bathgate, Xiao-Jun Du, Chrishan Samuel

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40 Citations (Scopus)

Abstract

In this study, we determined the effects of relaxin and estrogen deficiency and estrogen replacement therapy (ERT) on the cardiac, renal, and pulmonary phenotypes of female relaxin gene knockout (Rln1-/-) and age-matched wild-type (Rln1+/+) mice. One-month-old Rln1+/+ and Rln1-/- mice were bilaterally ovariectomized or sham-operated and aged until 9 or 12 months. A subgroup of ovariectomized mice received ERT from 9 to 12 months of age. At the appropriate time points, heart, kidney, and lung tissues from these mice were collected and analyzed for changes in organ fibrosis, hypertrophy, and airway thickening. Neither ovariectomy nor ERT had any effect on cardiac or renal collagen concentration in all groups studied. In contrast, total lung collagen concentration and airway subepithelial collagen deposition were significantly increased in ovariectomized Rln1+/+ mice (P
Original languageEnglish
Pages (from-to)5575 - 5583
Number of pages9
JournalEndocrinology
Volume147
Issue number12
DOIs
Publication statusPublished - 2006
Externally publishedYes

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