Abstract
OBJECTIVE: Endometriosis is a common and painful condition characterised by the formation of endometrial lesions within the peritoneal cavity. Previous studies have suggested a role for hedgehog signalling in the pathogenesis of endometriosis. We investigated the role of hedgehog signalling in the establishment of endometriosis lesions using 5E1, a hedgehog ligand neutralising antibody, and a mouse model of endometriosis. To mimic the initiation of endometriosis by retrograde menstruation, which is believed to occur in humans, donor mice underwent an artificial menstruation protocol. Fragments of menstrual endometrium were injected into the peritoneal cavity of estrogen primed recipients. Recipients received twice weekly injections of 5E1 or an isotype matched control antibody for three weeks. Lesions were collected and analysed for markers of epithelium, proliferation and apoptosis by immunofluorescence microscopy. RESULTS: Treatment with 5E1 reduced the number of lesions found on the mesentery. No significant changes were found in the size of lesions, abundance of endometrial epithelial cells, proliferation or apoptosis.
Original language | English |
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Article number | 454 |
Number of pages | 6 |
Journal | BMC Research Notes |
Volume | 13 |
Issue number | 1 |
DOIs | |
Publication status | Published - 25 Sep 2020 |
Keywords
- Endometriosis
- Endometrium
- Hedgehog signalling
- Mouse model
Equipment
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Animal Research Platform (MARP)
Tanya Lennane (Manager)
Faculty of Medicine Nursing and Health Sciences Research PlatformsFacility/equipment: Facility
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Histology Platform
Camilla Cohen (Manager)
Faculty of Medicine Nursing and Health Sciences Research PlatformsFacility/equipment: Facility
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MHTP Cell Therapies Platform
Gordon McPhee (Manager)
Hudson Institute - Department of Molecular and Translational ScienceFacility/equipment: Facility