Projects per year
Abstract
Observational studies suggest a link between vitamin D and the composition of the gut microbiome, but there is little evidence from randomized controlled trials of vitamin D supplementation. We analyzed data from the D-Health Trial, a randomized, double-blind, placebo-controlled trial. We recruited 21,315 Australians aged 60-84 y and randomized them to 60,000 IU of vitamin D3 or placebo monthly for 5 y. Stool samples were collected from a sample of 835 participants (417 in the placebo and 418 in the vitamin D group) approximately 5 y after randomization. We characterized the gut microbiome using 16S rRNA gene sequencing. We used linear regression to compare alpha diversity indices (i.e. Shannon index (primary outcome), richness, inverse Simpson index), and the ratio of Firmicutes to Bacteroidetes between the two groups. We analyzed between-sample (beta) diversity (i.e. Bray Curtis distance and UniFrac index) using principal coordinate analysis and used PERMANOVA to test for significant clustering according to randomization group. We also assessed the difference in the abundance of the 20 most abundant genera between the two groups using negative binomial regression model with adjustment for multiple testing. Approximately half the participants included in this analysis were women (mean age 69.4 y). Vitamin D supplementation did not alter the Shannon diversity index (mean 3.51 versus 3.52 in the placebo and vitamin D groups, respectively, p = 0.50). Similarly, there was little difference between the groups for other alpha diversity indices, the abundance of different genera, and the Firmicutes-to-Bacteroidetes ratio. We did not observe clustering of bacterial communities according to randomization group. In conlusion, monthly doses of 60,000 IU of vitamin D supplementation for 5 y did not alter the composition of the gut microbiome in older Australians.
Original language | English |
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Article number | 2221429 |
Number of pages | 13 |
Journal | Gut Microbes |
Volume | 15 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1 Jan 2023 |
Keywords
- Gut microbiome
- randomized controlled trial
- vitamin D supplementation
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Increasing osteoporosis treatment uptake
National Health and Medical Research Council (NHMRC) (Australia)
1/01/21 → 31/12/25
Project: Research
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D-Health: The D-Health Trial: a large-scale population-based trial of vitamin D supplementation for improving the health of older adults.
Neale, R., English, D. R., Venn, A. J., Armstrong, B., Ebeling, P., Kimlin, M. G., Van Der Pols, J. C. & Wockner, L. F.
National Health and Medical Research Council (NHMRC) (Australia)
1/01/17 → 31/12/21
Project: Research