The effect of norethynodrel-mestranol pretreatment on hepatic metabolism of 7,12-dimethylbenz(a)anthracene in rats and hamsters

Paul J. Hertzog; Verna Ilacqua; Mario Rustia; Ralph Gingell

doi:10.1016/0041-008X(80)90204-5

The effect of norethynodrel-mestranol pretreatment on hepatic metabolism of 7,12-dimethylbenz(a)anthracene in rats and hamsters

Paul J. Hertzog, Verna Ilacqua, Mario Rustia, Ralph Gingell

Research output: Contribution to journal › Article › Research › peer-review

4 Citations (Scopus)

Abstract

The effect of norethynodrelmestranol ("Enovid") pretreatment on the metabolism of 7,12-dimethylbenz(a)anthracene (DMBA) was examined in vivo in rats and hamsters, and in vitro in hamsters. "Enovid" pretreatment caused a decrease in bile flow, and increased by 44% the amount of DMBA metabolites excreted in the bile of rats; however, such effects were not found in hamsters. In vitro metabolism of DMBA by hamster liver 10,000g homogenates was also not modified by "Enovid" pretreatment. Aroclor and 3-methylcholanthrene pretreatments were used as positive controls for induction. Aroclor induced in vitro DMBA metabolism in hamster liver homogenates, whereas 3-methylcholanthrene unexpectedly had no effect. Results for in vitro benzo(a)pyrene metabolism were similar to those for DMBA. These studies suggest that the reported enhancement by Enovid E pretreatment of DMBA mammary carcinogenesis in the hamster is not mediated by an effect on overall hepatic metabolic activation of DMBA.

Original language	English
Pages (from-to)	340-347
Number of pages	8
Journal	Toxicology and Applied Pharmacology
Volume	54
Issue number	2
DOIs	https://doi.org/10.1016/0041-008X(80)90204-5
Publication status	Published - 30 Jun 1980
Externally published	Yes

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title = "The effect of norethynodrel-mestranol pretreatment on hepatic metabolism of 7,12-dimethylbenz(a)anthracene in rats and hamsters",

abstract = "The effect of norethynodrelmestranol ({"}Enovid{"}) pretreatment on the metabolism of 7,12-dimethylbenz(a)anthracene (DMBA) was examined in vivo in rats and hamsters, and in vitro in hamsters. {"}Enovid{"} pretreatment caused a decrease in bile flow, and increased by 44\% the amount of DMBA metabolites excreted in the bile of rats; however, such effects were not found in hamsters. In vitro metabolism of DMBA by hamster liver 10,000g homogenates was also not modified by {"}Enovid{"} pretreatment. Aroclor and 3-methylcholanthrene pretreatments were used as positive controls for induction. Aroclor induced in vitro DMBA metabolism in hamster liver homogenates, whereas 3-methylcholanthrene unexpectedly had no effect. Results for in vitro benzo(a)pyrene metabolism were similar to those for DMBA. These studies suggest that the reported enhancement by Enovid E pretreatment of DMBA mammary carcinogenesis in the hamster is not mediated by an effect on overall hepatic metabolic activation of DMBA.",

author = "Hertzog, \{Paul J.\} and Verna Ilacqua and Mario Rustia and Ralph Gingell",

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T1 - The effect of norethynodrel-mestranol pretreatment on hepatic metabolism of 7,12-dimethylbenz(a)anthracene in rats and hamsters

AU - Hertzog, Paul J.

AU - Ilacqua, Verna

AU - Rustia, Mario

AU - Gingell, Ralph

PY - 1980/6/30

Y1 - 1980/6/30

N2 - The effect of norethynodrelmestranol ("Enovid") pretreatment on the metabolism of 7,12-dimethylbenz(a)anthracene (DMBA) was examined in vivo in rats and hamsters, and in vitro in hamsters. "Enovid" pretreatment caused a decrease in bile flow, and increased by 44% the amount of DMBA metabolites excreted in the bile of rats; however, such effects were not found in hamsters. In vitro metabolism of DMBA by hamster liver 10,000g homogenates was also not modified by "Enovid" pretreatment. Aroclor and 3-methylcholanthrene pretreatments were used as positive controls for induction. Aroclor induced in vitro DMBA metabolism in hamster liver homogenates, whereas 3-methylcholanthrene unexpectedly had no effect. Results for in vitro benzo(a)pyrene metabolism were similar to those for DMBA. These studies suggest that the reported enhancement by Enovid E pretreatment of DMBA mammary carcinogenesis in the hamster is not mediated by an effect on overall hepatic metabolic activation of DMBA.

AB - The effect of norethynodrelmestranol ("Enovid") pretreatment on the metabolism of 7,12-dimethylbenz(a)anthracene (DMBA) was examined in vivo in rats and hamsters, and in vitro in hamsters. "Enovid" pretreatment caused a decrease in bile flow, and increased by 44% the amount of DMBA metabolites excreted in the bile of rats; however, such effects were not found in hamsters. In vitro metabolism of DMBA by hamster liver 10,000g homogenates was also not modified by "Enovid" pretreatment. Aroclor and 3-methylcholanthrene pretreatments were used as positive controls for induction. Aroclor induced in vitro DMBA metabolism in hamster liver homogenates, whereas 3-methylcholanthrene unexpectedly had no effect. Results for in vitro benzo(a)pyrene metabolism were similar to those for DMBA. These studies suggest that the reported enhancement by Enovid E pretreatment of DMBA mammary carcinogenesis in the hamster is not mediated by an effect on overall hepatic metabolic activation of DMBA.

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DO - 10.1016/0041-008X(80)90204-5

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SN - 0041-008X

VL - 54

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JF - Toxicology and Applied Pharmacology

IS - 2

ER -

The effect of norethynodrel-mestranol pretreatment on hepatic metabolism of 7,12-dimethylbenz(a)anthracene in rats and hamsters

Abstract

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