The effect of norethynodrel-mestranol pretreatment on hepatic metabolism of 7,12-dimethylbenz(a)anthracene in rats and hamsters

Paul J. Hertzog, Verna Ilacqua, Mario Rustia, Ralph Gingell

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3 Citations (Scopus)

Abstract

The effect of norethynodrelmestranol ("Enovid") pretreatment on the metabolism of 7,12-dimethylbenz(a)anthracene (DMBA) was examined in vivo in rats and hamsters, and in vitro in hamsters. "Enovid" pretreatment caused a decrease in bile flow, and increased by 44% the amount of DMBA metabolites excreted in the bile of rats; however, such effects were not found in hamsters. In vitro metabolism of DMBA by hamster liver 10,000g homogenates was also not modified by "Enovid" pretreatment. Aroclor and 3-methylcholanthrene pretreatments were used as positive controls for induction. Aroclor induced in vitro DMBA metabolism in hamster liver homogenates, whereas 3-methylcholanthrene unexpectedly had no effect. Results for in vitro benzo(a)pyrene metabolism were similar to those for DMBA. These studies suggest that the reported enhancement by Enovid E pretreatment of DMBA mammary carcinogenesis in the hamster is not mediated by an effect on overall hepatic metabolic activation of DMBA.

Original languageEnglish
Pages (from-to)340-347
Number of pages8
JournalToxicology and Applied Pharmacology
Volume54
Issue number2
DOIs
Publication statusPublished - 30 Jun 1980
Externally publishedYes

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