The effect of gestational age on angiogenic gene expression in the rat placenta

Kanchan Vaswani, Melissa W C Hum, Hsiu-Wen Chan, Jennifer Ryan, Ryan Wood-Bradley, Marloes D Nitert, Murray D Mitchell, James Armitage, Gregory E Rice

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Abstract

The placenta plays a central role in determining the outcome of pregnancy. It undergoes changes during gestation as the fetus develops and as demands for energy substrate transfer and gas exchange increase. The molecular mechanisms that coordinate these changes have yet to be fully elucidated. The study performed a large scale screen of the transcriptome of the rat placenta throughout mid-late gestation (E14.25-E20) with emphasis on characterizing gestational age associated changes in the expression of genes invoved in angiogenic pathways. Sprague Dawley dams were sacrificed at E14.25, E15.25, E17.25 and E20 (n = 6 per group) and RNA was isolated from one placenta per dam. Changes in placental gene expression were identifed using Illumina Rat Ref-12 Expression BeadChip Microarrays. Differentially expressed genes (>2-fold change,
Original languageEnglish
Article numbere83762
Number of pages11
JournalPLoS ONE
Volume8
Issue number12
DOIs
Publication statusPublished - 2013

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