The effect of exercise on the skeletal muscle phospholipidome of rats fed a high-fat diet

Todd Mitchell, Nigel Turner, Paul L Else, Anthony J Hulbert, John A Hawley, Jong Sam Lee, Clinton R Bruce, Stephen J Blanksby

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)


The aim of this study was to examine the effect of endurance raining on skeletal muscle phospholipid molecular species from high-fat fed rats. Twelve female Sprague-Dawley rats were fed a high-fat diet (78.1 energy). The rats were randomly divided into two groups, a sedentary control group and a trained group (125 min of treadmill running at 8 m/min, 4 days/wk for 4 weeks). Forty-eight hours after their last training bout phospholipids were extracted from the red and white vastus lateralis and analyzed by electrospray-ionization mass spectrometry. Exercise training was associated with significant alterations in the relative abundance of a number of phospholipid molecular species. These changes were more prominent in red vastus lateralis than white vastus lateralis. The largest observed change was an increase of 30 in the abundance of 1-palmitoyl-2-linoleoyl phosphatidylcholine ions in oxidative fibers. Reductions in the relative abundance of a number of phospholipids containing long-chain n-3 polyunsaturated fatty acids were also observed. These data suggest a possible reduction in phospholipid remodeling in the trained animals. This results in a decrease in the phospholipid n-3 to n-6 ratio that may in turn influence endurance capacity.
Original languageEnglish
Pages (from-to)3954 - 3964
Number of pages11
JournalInternational Journal of Molecular Sciences
Issue number10
Publication statusPublished - 2010
Externally publishedYes

Cite this