To assess possible functional effects of the procedures involved in freezing/thawing of mammalian embryos, uptake of3H-glycine and125I-BSA (bovine serum albumin) was measured in mouse two-cell embryos and morulae before and after freezing. None of the procedures of dehydration, rehydration, or freezing affected glycine uptake significantly. Earlier results for uptake of125I-BSA by fresh embryos which indicated an increase in endocytic activity between morula and blastocyst stages were confirmed, and hydrolysis of endocytosed125I-BSA by morulae but not two-cell embryos was observed. Only 53 ±9% of two-cell embryos but 83±7% of morulae were apparently normal after freezing and thawing. Apparently normal frozen/thawed two-cell embryos were twice as active in125I-BSA uptake as controls. This was not the case for morulae. The results suggest that freezing/ thawing effects endocytotic processes in two-cell embryos but not morulae.
|Number of pages||6|
|Journal||Journal of In Vitro Fertilization and Embryo Transfer|
|Publication status||Published - 1 Apr 1988|
- amino acid uptake
- mouse embryo