The draft genome sequence of Actinokineospora bangkokensis 44EHWT reveals the biosynthetic pathway of the antifungal thailandin compounds with unusual butylmalonyl-CoA extender units

Anja Greule; Bungonsiri Intra; Stephan Flemming; Marcel G E Rommel; Watanalai Panbangred; Andreas Bechthold

doi:10.3390/molecules21111607

The draft genome sequence of Actinokineospora bangkokensis 44EHW^T reveals the biosynthetic pathway of the antifungal thailandin compounds with unusual butylmalonyl-CoA extender units

Anja Greule, Bungonsiri Intra, Stephan Flemming, Marcel G E Rommel, Watanalai Panbangred, Andreas Bechthold

Research output: Contribution to journal › Article › Research › peer-review

5 Citations (Scopus)

Abstract

We report the draft genome sequence of Actinokineospora bangkokensis 44EHW^T, the producer of the antifungal polyene compounds, thailandins A and B. The sequence contains 7.45 Mb, 74.1% GC content and 35 putative gene clusters for the biosynthesis of secondary metabolites. There are three gene clusters encoding large polyketide synthases of type I. Annotation of the ORF functions and targeted gene disruption enabled us to identify the cluster for thailandin biosynthesis. We propose a plausible biosynthetic pathway for thailandin, where the unusual butylmalonyl-CoA extender unit is incorporated and results in an untypical side chain.

Original language	English
Article number	1607
Number of pages	16
Journal	Molecules
Volume	21
Issue number	11
DOIs	https://doi.org/10.3390/molecules21111607
Publication status	Published - 23 Nov 2016
Externally published	Yes

Keywords

Actinokineospora bangkokensis
Biosynthetic gene cluster
Butylmalonyl-CoA
Draft genome sequence
Genome mining
Polyene
Thailandin

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10.3390/molecules21111607

24041212-oaFinal published version, 2.45 MB

Cite this

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title = "The draft genome sequence of Actinokineospora bangkokensis 44EHWT reveals the biosynthetic pathway of the antifungal thailandin compounds with unusual butylmalonyl-CoA extender units",

abstract = "We report the draft genome sequence of Actinokineospora bangkokensis 44EHWT, the producer of the antifungal polyene compounds, thailandins A and B. The sequence contains 7.45 Mb, 74.1% GC content and 35 putative gene clusters for the biosynthesis of secondary metabolites. There are three gene clusters encoding large polyketide synthases of type I. Annotation of the ORF functions and targeted gene disruption enabled us to identify the cluster for thailandin biosynthesis. We propose a plausible biosynthetic pathway for thailandin, where the unusual butylmalonyl-CoA extender unit is incorporated and results in an untypical side chain.",

keywords = "Actinokineospora bangkokensis, Biosynthetic gene cluster, Butylmalonyl-CoA, Draft genome sequence, Genome mining, Polyene, Thailandin",

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The draft genome sequence of Actinokineospora bangkokensis 44EHW^T reveals the biosynthetic pathway of the antifungal thailandin compounds with unusual butylmalonyl-CoA extender units. / Greule, Anja; Intra, Bungonsiri; Flemming, Stephan et al.
In: Molecules, Vol. 21, No. 11, 1607, 23.11.2016.

Research output: Contribution to journal › Article › Research › peer-review

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T1 - The draft genome sequence of Actinokineospora bangkokensis 44EHWT reveals the biosynthetic pathway of the antifungal thailandin compounds with unusual butylmalonyl-CoA extender units

AU - Greule, Anja

AU - Intra, Bungonsiri

AU - Flemming, Stephan

AU - Rommel, Marcel G E

AU - Panbangred, Watanalai

AU - Bechthold, Andreas

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AB - We report the draft genome sequence of Actinokineospora bangkokensis 44EHWT, the producer of the antifungal polyene compounds, thailandins A and B. The sequence contains 7.45 Mb, 74.1% GC content and 35 putative gene clusters for the biosynthesis of secondary metabolites. There are three gene clusters encoding large polyketide synthases of type I. Annotation of the ORF functions and targeted gene disruption enabled us to identify the cluster for thailandin biosynthesis. We propose a plausible biosynthetic pathway for thailandin, where the unusual butylmalonyl-CoA extender unit is incorporated and results in an untypical side chain.

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KW - Biosynthetic gene cluster

KW - Butylmalonyl-CoA

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