TY - JOUR
T1 - The distribution of β-adrenoceptors in dog kidney
T2 - an autoradiographic analysis
AU - Law, Robert
AU - Summers, Roger J.
PY - 1987/8/4
Y1 - 1987/8/4
N2 - The distribution of β-adrenoceptors in slide-mounted dog kidney sections was determined using the radioligand (-)-[125I]cyanopindolol ((-)-[125I]CYP) and autoradiography. Using conditions designed to prevent (-)-[125I]CYP binding to non-β-adrenoceptor sites, biochemical studies revealed that (-)-[125I]CYP binding equilibrated within 150 min (K1 = 3.2 × 108 M-1 min-1), was saturable (KD = 30.72 ± 2.96 pM; Bmax = 0.57 ± 0.03 fmol/section, n = 4) and stereoselective with respect to the stereoisomers of propranolol and pindolol. Delineation of β-adrenoceptor subtypes with the selective β1-adrenoceptor antagonist betaxolol and β2-adrenoceptor antagonist ICI 118,551 demonstrated that the proportions of β1-:β2-adrenoceptors was between 1:6 and 1:11. Autoradiographic studies showed that β1-adrenoceptors were localized on the juxtaglomerular apparatus and glomwruli, while β2-adrenoceptors were localized on medullary rays. The distribution of β-adrenoceptors with respect to renal function in the dog kidney is discussed.
AB - The distribution of β-adrenoceptors in slide-mounted dog kidney sections was determined using the radioligand (-)-[125I]cyanopindolol ((-)-[125I]CYP) and autoradiography. Using conditions designed to prevent (-)-[125I]CYP binding to non-β-adrenoceptor sites, biochemical studies revealed that (-)-[125I]CYP binding equilibrated within 150 min (K1 = 3.2 × 108 M-1 min-1), was saturable (KD = 30.72 ± 2.96 pM; Bmax = 0.57 ± 0.03 fmol/section, n = 4) and stereoselective with respect to the stereoisomers of propranolol and pindolol. Delineation of β-adrenoceptor subtypes with the selective β1-adrenoceptor antagonist betaxolol and β2-adrenoceptor antagonist ICI 118,551 demonstrated that the proportions of β1-:β2-adrenoceptors was between 1:6 and 1:11. Autoradiographic studies showed that β1-adrenoceptors were localized on the juxtaglomerular apparatus and glomwruli, while β2-adrenoceptors were localized on medullary rays. The distribution of β-adrenoceptors with respect to renal function in the dog kidney is discussed.
KW - (-)-[I]Cyanopindolol
KW - (Dog)
KW - Autoradiography
KW - Kidney
KW - β-Adrenoceptors
UR - http://www.scopus.com/inward/record.url?scp=0023238462&partnerID=8YFLogxK
U2 - 10.1016/0014-2999(87)90627-3
DO - 10.1016/0014-2999(87)90627-3
M3 - Article
C2 - 2887444
AN - SCOPUS:0023238462
SN - 0014-2999
VL - 140
SP - 1
EP - 11
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 1
ER -