The development of functional B lymphocytes in conditional PU.1 knock-out mice

Matthew Polli, Aleksandar Dakic, Amanda Light, Li Wu, David M. Tarlinton, Stephen L. Nutt

Research output: Contribution to journalArticleResearchpeer-review

60 Citations (Scopus)

Abstract

An abundance of research has entrenched the view that the Ets domain containing transcription factor PU.1 is fundamental to the development and function of B lymphocytes. In this study, we have made use of a conditional PU.1 allele to test this notion. Complete deletion of PU.1 resulted in the loss of B cells and all other lineage-positive cells in the fetal liver and death between E18.5 and birth; however, specific deletion of PU.1 in the B lineage had no effect on B-cell development. Furthermore, deletion of PU.1 in B cells did not compromise their ability to establish and maintain an immune response. An increased level of apoptosis was observed in vitro upon B-cell receptor (BCR) cross-linking; however, this was partially rescued by interleukin-4 (1L-4). These findings suggest that PU.1 is not essential for the development of functional B lymphocytes beyond the pre-B stage.

Original languageEnglish
Pages (from-to)2083-2090
Number of pages8
JournalBlood
Volume106
Issue number6
DOIs
Publication statusPublished - 15 Sep 2005

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