The development of an enzyme-linked immunosorbent assay (ELISA) for cephalexin

Edward H. Kachab, Wen Yang Wu, Colin B. Chapman

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10 Citations (Scopus)

Abstract

Cephalexin was structurally modified by the attachment of a spacer at the carboxylic acid through which it was subsequently covalently attached to BSA. This method permitted the molecule to be attached without cleavage of the β-lactam ring giving a conjugate distinct from previously described immunogenic preparations of penicillins and cephalosporins. This approach required the development of a novel spacer molecule, and its synthesis and characterisation are reported. Rabbits were used to raise antisera and the antibodies produced were characterised with respect to their reactivity with cephalexin and various analogues, other cephalosporins, and a number of penicillins.

Original languageEnglish
Pages (from-to)33-41
Number of pages9
JournalJournal of Immunological Methods
Volume147
Issue number1
DOIs
Publication statusPublished - 1 Jan 1992

Keywords

  • 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide
  • 2,2′-azino-bis(3-ethylbenzthiazoline-6-sul-phonic acid)
  • 7-ADCA
  • 7-aminodesacetoxycephalosporanic acid
  • ABTS
  • bovine serum albumin
  • BSA
  • Competitive inhibition
  • Conjugate
  • EDC
  • ELISA
  • Hapten
  • horseradish peroxidase
  • HRP
  • keyhole limpet haemo-cyanin
  • KLH
  • N-hydroxysulphosuccinimide
  • OD
  • optical density
  • PBS
  • phosphate-buffered saline
  • Spacer
  • sulpho-NHS

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