The dengue virus M protein localises to the endoplasmic reticulum and forms oligomers

Sook-San Wong, Gholamreza Haqshenas, Eric J Gowans, Jason Mackenzie

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7 Citations (Scopus)

Abstract

The dengue virus membrane (M) protein is a key component of the mature virion. Here, we characterised the cellular behaviour of M using a recombinant protein construct to understand its inherent properties. Using confocal microscopy, we showed that M and its intracellular precursor, prM, localised to the endoplasmic reticulum. M protein was also detected on the cell surface and secreted, suggesting that M can enter the secretory pathway. In addition, cross-linking studies showed that M can form dimers and tetramers. These findings suggest that M behaves as a secretory protein analogous to the major envelope protein E.
Original languageEnglish
Pages (from-to)1032 - 1037
Number of pages6
JournalFEBS Letters
Volume586
Issue number7
DOIs
Publication statusPublished - 2012

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