The dengue virus membrane (M) protein is a key component of the mature virion. Here, we characterised the cellular behaviour of M using a recombinant protein construct to understand its inherent properties. Using confocal microscopy, we showed that M and its intracellular precursor, prM, localised to the endoplasmic reticulum. M protein was also detected on the cell surface and secreted, suggesting that M can enter the secretory pathway. In addition, cross-linking studies showed that M can form dimers and tetramers. These findings suggest that M behaves as a secretory protein analogous to the major envelope protein E.
Wong, S-S., Haqshenas, G., Gowans, E. J., & Mackenzie, J. (2012). The dengue virus M protein localises to the endoplasmic reticulum and forms oligomers. FEBS Letters, 586(7), 1032 - 1037. https://doi.org/10.1016/j.febslet.2012.02.047