TY - JOUR
T1 - The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II
AU - Elmlund, Hans
AU - Baraznenok, Vera
AU - Lindahl, Martin
AU - Samuelsen, Camilla O.
AU - Koeck, Philip J.B.
AU - Holmberg, Steen
AU - Hebert, Hans
AU - Gustafsson, Claes M.
PY - 2006/10/24
Y1 - 2006/10/24
N2 - CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.
AB - CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.
KW - Electron microscopy
KW - Protein structure
KW - Schizosaccharomyces pombe
KW - Transcription
KW - Yeast
UR - http://www.scopus.com/inward/record.url?scp=33750481873&partnerID=8YFLogxK
U2 - 10.1073/pnas.0607483103
DO - 10.1073/pnas.0607483103
M3 - Article
C2 - 17043218
AN - SCOPUS:33750481873
SN - 0027-8424
VL - 103
SP - 15788
EP - 15793
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 43
ER -