The cross-priming APC requires a rel-dependent signal to induce CTL

Justine D. Mintern, Gabrielle Belz, Steve Gerondakis, Francis R. Carbone, William R. Heath

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15 Citations (Scopus)


Induction of OVA-specific CTL by cross-priming requires help from CD4 T cells, which use CD154 to signal CD40 on the APC. To further dissect the molecular pathways involved in cross-priming, we examined the role of Rel, an NF-κB family member. c-rel-/- mice failed to generate OVA-specific CTL by cross-priming, but could induce CTL to HSV-1. Using chimeric mice, Rel expression was shown to be required by the APC, but not by the T cells. Notably, the deficiency in Rel could be overcome by triggering CD40, implying that the APC required Rel before receipt of the CD40 signal. These data suggest that the cross-priming APC must receive two signals before it can stimulate CTL. The first signal is Rel dependent and is required before activation of CD4 helper T cells, which then deliver the second signal using CD154 to trigger CD40.

Original languageEnglish
Pages (from-to)3283-3287
Number of pages5
JournalJournal of Immunology
Issue number7
Publication statusPublished - 1 Apr 2002
Externally publishedYes

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