The Copper bis(thiosemicarbazone) Complex CuII(atsm) Is Protective Against Cerebral Ischemia Through Modulation of the Inflammatory Milieu

Mikko T. Huuskonen, Qing zhang Tuo, Sanna Loppi, Hiramani Dhungana, Paula Korhonen, Lachlan E. McInnes, Paul S. Donnelly, Alexandra Grubman, Sara Wojciechowski, Katarina Lejavova, Yuriy Pomeshchik, Laura Periviita, Lotta Kosonen, Martina Giordano, Frederick R. Walker, Rong Liu, Ashley I. Bush, Jari Koistinaho, Tarja Malm, Anthony R. White & 2 others Peng Lei, Katja M. Kanninen

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Developing new therapies for stroke is urgently needed, as this disease is the leading cause of death and disability worldwide, and the existing treatment is only available for a small subset of patients. The interruption of blood flow to the brain during ischemic stroke launches multiple immune responses, characterized by infiltration of peripheral immune cells, the activation of brain microglial cells, and the accumulation of immune mediators. Copper is an essential trace element that is required for many critical processes in the brain. Copper homeostasis is disturbed in chronic neurodegenerative diseases and altered in stroke patients, and targeted copper delivery has been shown to be protective against chronic neurodegeneration. This study was undertaken to assess whether the copper bis(thiosemicarbazone) complex, CuII(atsm), is beneficial in acute brain injury, in preclinical mouse models of ischemic stroke. We demonstrate that the copper complex CuII(atsm) protects neurons from excitotoxicity and N2a cells from OGD in vitro, and is protective in permanent and transient ischemia models in mice as measured by functional outcome and lesion size. Copper delivery in the ischemic brains modulates the inflammatory response, specifically affecting the myeloid cells. It reduces CD45 and Iba1 immunoreactivity, and alters the morphology of Iba1 positive cells in the ischemic brain. CuII(atsm) also protects endogenous microglia against ischemic insult and reduces the proportion of invading monocytes. These results demonstrate that the copper complex CuII(atsm) is an inflammation-modulating compound with high therapeutic potential in stroke and is a strong candidate for the development of therapies for acute brain injury.

Original languageEnglish
Pages (from-to)519-532
Number of pages14
JournalNeurotherapeutics
Volume14
Issue number2
DOIs
Publication statusPublished - Apr 2017
Externally publishedYes

Keywords

  • CuII(atsm)
  • stroke
  • microglia
  • neuron
  • neuroinflammation

Cite this

Huuskonen, M. T., Tuo, Q. Z., Loppi, S., Dhungana, H., Korhonen, P., McInnes, L. E., ... Kanninen, K. M. (2017). The Copper bis(thiosemicarbazone) Complex CuII(atsm) Is Protective Against Cerebral Ischemia Through Modulation of the Inflammatory Milieu. Neurotherapeutics, 14(2), 519-532. https://doi.org/10.1007/s13311-016-0504-9
Huuskonen, Mikko T. ; Tuo, Qing zhang ; Loppi, Sanna ; Dhungana, Hiramani ; Korhonen, Paula ; McInnes, Lachlan E. ; Donnelly, Paul S. ; Grubman, Alexandra ; Wojciechowski, Sara ; Lejavova, Katarina ; Pomeshchik, Yuriy ; Periviita, Laura ; Kosonen, Lotta ; Giordano, Martina ; Walker, Frederick R. ; Liu, Rong ; Bush, Ashley I. ; Koistinaho, Jari ; Malm, Tarja ; White, Anthony R. ; Lei, Peng ; Kanninen, Katja M. / The Copper bis(thiosemicarbazone) Complex CuII(atsm) Is Protective Against Cerebral Ischemia Through Modulation of the Inflammatory Milieu. In: Neurotherapeutics. 2017 ; Vol. 14, No. 2. pp. 519-532.
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abstract = "Developing new therapies for stroke is urgently needed, as this disease is the leading cause of death and disability worldwide, and the existing treatment is only available for a small subset of patients. The interruption of blood flow to the brain during ischemic stroke launches multiple immune responses, characterized by infiltration of peripheral immune cells, the activation of brain microglial cells, and the accumulation of immune mediators. Copper is an essential trace element that is required for many critical processes in the brain. Copper homeostasis is disturbed in chronic neurodegenerative diseases and altered in stroke patients, and targeted copper delivery has been shown to be protective against chronic neurodegeneration. This study was undertaken to assess whether the copper bis(thiosemicarbazone) complex, CuII(atsm), is beneficial in acute brain injury, in preclinical mouse models of ischemic stroke. We demonstrate that the copper complex CuII(atsm) protects neurons from excitotoxicity and N2a cells from OGD in vitro, and is protective in permanent and transient ischemia models in mice as measured by functional outcome and lesion size. Copper delivery in the ischemic brains modulates the inflammatory response, specifically affecting the myeloid cells. It reduces CD45 and Iba1 immunoreactivity, and alters the morphology of Iba1 positive cells in the ischemic brain. CuII(atsm) also protects endogenous microglia against ischemic insult and reduces the proportion of invading monocytes. These results demonstrate that the copper complex CuII(atsm) is an inflammation-modulating compound with high therapeutic potential in stroke and is a strong candidate for the development of therapies for acute brain injury.",
keywords = "CuII(atsm), stroke, microglia, neuron, neuroinflammation",
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Huuskonen, MT, Tuo, QZ, Loppi, S, Dhungana, H, Korhonen, P, McInnes, LE, Donnelly, PS, Grubman, A, Wojciechowski, S, Lejavova, K, Pomeshchik, Y, Periviita, L, Kosonen, L, Giordano, M, Walker, FR, Liu, R, Bush, AI, Koistinaho, J, Malm, T, White, AR, Lei, P & Kanninen, KM 2017, 'The Copper bis(thiosemicarbazone) Complex CuII(atsm) Is Protective Against Cerebral Ischemia Through Modulation of the Inflammatory Milieu' Neurotherapeutics, vol. 14, no. 2, pp. 519-532. https://doi.org/10.1007/s13311-016-0504-9

The Copper bis(thiosemicarbazone) Complex CuII(atsm) Is Protective Against Cerebral Ischemia Through Modulation of the Inflammatory Milieu. / Huuskonen, Mikko T.; Tuo, Qing zhang; Loppi, Sanna; Dhungana, Hiramani; Korhonen, Paula; McInnes, Lachlan E.; Donnelly, Paul S.; Grubman, Alexandra; Wojciechowski, Sara; Lejavova, Katarina; Pomeshchik, Yuriy; Periviita, Laura; Kosonen, Lotta; Giordano, Martina; Walker, Frederick R.; Liu, Rong; Bush, Ashley I.; Koistinaho, Jari; Malm, Tarja; White, Anthony R.; Lei, Peng; Kanninen, Katja M.

In: Neurotherapeutics, Vol. 14, No. 2, 04.2017, p. 519-532.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - The Copper bis(thiosemicarbazone) Complex CuII(atsm) Is Protective Against Cerebral Ischemia Through Modulation of the Inflammatory Milieu

AU - Huuskonen, Mikko T.

AU - Tuo, Qing zhang

AU - Loppi, Sanna

AU - Dhungana, Hiramani

AU - Korhonen, Paula

AU - McInnes, Lachlan E.

AU - Donnelly, Paul S.

AU - Grubman, Alexandra

AU - Wojciechowski, Sara

AU - Lejavova, Katarina

AU - Pomeshchik, Yuriy

AU - Periviita, Laura

AU - Kosonen, Lotta

AU - Giordano, Martina

AU - Walker, Frederick R.

AU - Liu, Rong

AU - Bush, Ashley I.

AU - Koistinaho, Jari

AU - Malm, Tarja

AU - White, Anthony R.

AU - Lei, Peng

AU - Kanninen, Katja M.

PY - 2017/4

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N2 - Developing new therapies for stroke is urgently needed, as this disease is the leading cause of death and disability worldwide, and the existing treatment is only available for a small subset of patients. The interruption of blood flow to the brain during ischemic stroke launches multiple immune responses, characterized by infiltration of peripheral immune cells, the activation of brain microglial cells, and the accumulation of immune mediators. Copper is an essential trace element that is required for many critical processes in the brain. Copper homeostasis is disturbed in chronic neurodegenerative diseases and altered in stroke patients, and targeted copper delivery has been shown to be protective against chronic neurodegeneration. This study was undertaken to assess whether the copper bis(thiosemicarbazone) complex, CuII(atsm), is beneficial in acute brain injury, in preclinical mouse models of ischemic stroke. We demonstrate that the copper complex CuII(atsm) protects neurons from excitotoxicity and N2a cells from OGD in vitro, and is protective in permanent and transient ischemia models in mice as measured by functional outcome and lesion size. Copper delivery in the ischemic brains modulates the inflammatory response, specifically affecting the myeloid cells. It reduces CD45 and Iba1 immunoreactivity, and alters the morphology of Iba1 positive cells in the ischemic brain. CuII(atsm) also protects endogenous microglia against ischemic insult and reduces the proportion of invading monocytes. These results demonstrate that the copper complex CuII(atsm) is an inflammation-modulating compound with high therapeutic potential in stroke and is a strong candidate for the development of therapies for acute brain injury.

AB - Developing new therapies for stroke is urgently needed, as this disease is the leading cause of death and disability worldwide, and the existing treatment is only available for a small subset of patients. The interruption of blood flow to the brain during ischemic stroke launches multiple immune responses, characterized by infiltration of peripheral immune cells, the activation of brain microglial cells, and the accumulation of immune mediators. Copper is an essential trace element that is required for many critical processes in the brain. Copper homeostasis is disturbed in chronic neurodegenerative diseases and altered in stroke patients, and targeted copper delivery has been shown to be protective against chronic neurodegeneration. This study was undertaken to assess whether the copper bis(thiosemicarbazone) complex, CuII(atsm), is beneficial in acute brain injury, in preclinical mouse models of ischemic stroke. We demonstrate that the copper complex CuII(atsm) protects neurons from excitotoxicity and N2a cells from OGD in vitro, and is protective in permanent and transient ischemia models in mice as measured by functional outcome and lesion size. Copper delivery in the ischemic brains modulates the inflammatory response, specifically affecting the myeloid cells. It reduces CD45 and Iba1 immunoreactivity, and alters the morphology of Iba1 positive cells in the ischemic brain. CuII(atsm) also protects endogenous microglia against ischemic insult and reduces the proportion of invading monocytes. These results demonstrate that the copper complex CuII(atsm) is an inflammation-modulating compound with high therapeutic potential in stroke and is a strong candidate for the development of therapies for acute brain injury.

KW - CuII(atsm)

KW - stroke

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