The COOH-terminus of parathyroid hormone-related protein (PTHrP) interacts with β-arrestin 1B

Lindus A. Conlan, T. John Martin, Matthew T. Gillespie

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10 Citations (Scopus)

Abstract

Parathyroid hormone-related protein (PTHrP) has a diverse range of proposed biological activities participating in both extracellular and intracellular signaling. In order to identify candidate protein effectors, yeast two-hybrid screens were conducted using mature human PTHrP (residues 1-141) and the COOH-terminus (residues 107-141). Both PTHrP baits interacted with a β-arrestin 1B fragment, an important component of G-protein-coupled receptor desensitization and MAPK signaling. Co-immunoprecipitation, in vitro binding assays and colocalization experiments confirmed this interaction in human cells and this required residues 122-141 of PTHrP. These findings suggest that β-arrestin 1 acts as an effector for a novel function of PTHrP in cytoplasm.

Original languageEnglish
Pages (from-to)71-75
Number of pages5
JournalFEBS Letters
Volume527
Issue number1-3
DOIs
Publication statusPublished - 11 Sep 2002
Externally publishedYes

Keywords

  • β-Arrestin
  • Endosome
  • Parathyroid hormone-related protein
  • Yeast two-hybrid

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