The chloroplast β-subunit allows assembly of the Escherichia coli F0 portion of the energy transducing adenosine triphosphatase

A. L. Munn, P. R. Whitfeld, W. Bottomley, G. S. Hudson, D. A. Jans, F. Gibson, G. B. Cox

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Abstract

The effect of the expression of the chloroplast F1-ATPase β-subunit in two Escherichia coli β-subunit mutant strain was investigated. The amount of chloroplast β-subunit formed in E. coli was increased by introducing 'Shine-Dalgarno' sequence upstream from the translation start site. The chloroplast β-subunit was membranes bound but was unable to functionally replace the mutant β-subunit in a strain carrying the uncD409 However, in an E. coli mutant strain unable to form the β- and e{open}-subunits the presence of the chloroplast β-subunit enabled the assembly of a functional proton pore.

Original languageEnglish
Pages (from-to)82-88
Number of pages7
JournalBBA - Bioenergetics
Volume1060
Issue number1
DOIs
Publication statusPublished - 27 Sep 1991
Externally publishedYes

Keywords

  • (E. coli)
  • ATPase, FF
  • Beta-subunit
  • Chloroplast
  • β-Subunit

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