The chicken embryo as an efficient model to test the function of muscle fusion genes in amniotes

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The fusion of myoblasts into multinucleated myotubes is a crucial step of muscle growth during development and of muscle repair in the adult. While multiple genes were shown to play a role in this process, a vertebrate model where novel candidates can be tested and analyzed at high throughput and relative ease has been lacking. Here, we show that the early chicken embryo is a fast and robust model in which functional testing of muscle fusion candidate genes can be performed. We have used known modulators of muscle fusion, Rac1 and Cdc42, along with the in vivo electroporation of integrated, inducible vectors, to show that the chicken embryo is a suitable model in which their function can be tested and quantified. In addition to nuclei content, specific characteristics of the experimental model allow a fine characterization of additional morphological features that are nearly impossible to assess in other model organisms. This study should establish the chicken embryo as a cheap, reliable and powerful model in which novel vertebrate muscle fusion candidates can be evaluated.

Original languageEnglish
Article numbere0177681
Number of pages13
JournalPLoS ONE
Volume12
Issue number5
DOIs
Publication statusPublished - 16 May 2017

Keywords

  • cell fusion
  • embryos
  • muscle analysis
  • somites
  • muscle functions
  • muscle fibers
  • electroporation
  • membrane fusion

Cite this

@article{f405588623554d3db6ec41407b84673a,
title = "The chicken embryo as an efficient model to test the function of muscle fusion genes in amniotes",
abstract = "The fusion of myoblasts into multinucleated myotubes is a crucial step of muscle growth during development and of muscle repair in the adult. While multiple genes were shown to play a role in this process, a vertebrate model where novel candidates can be tested and analyzed at high throughput and relative ease has been lacking. Here, we show that the early chicken embryo is a fast and robust model in which functional testing of muscle fusion candidate genes can be performed. We have used known modulators of muscle fusion, Rac1 and Cdc42, along with the in vivo electroporation of integrated, inducible vectors, to show that the chicken embryo is a suitable model in which their function can be tested and quantified. In addition to nuclei content, specific characteristics of the experimental model allow a fine characterization of additional morphological features that are nearly impossible to assess in other model organisms. This study should establish the chicken embryo as a cheap, reliable and powerful model in which novel vertebrate muscle fusion candidates can be evaluated.",
keywords = "cell fusion, embryos, muscle analysis, somites, muscle functions, muscle fibers, electroporation, membrane fusion",
author = "Daniel Sieiro and Nad{\`e}ge V{\'e}ron and Christophe Marcelle",
year = "2017",
month = "5",
day = "16",
doi = "10.1371/journal.pone.0177681",
language = "English",
volume = "12",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",

}

The chicken embryo as an efficient model to test the function of muscle fusion genes in amniotes. / Sieiro, Daniel; Véron, Nadège; Marcelle, Christophe.

In: PLoS ONE, Vol. 12, No. 5, e0177681, 16.05.2017.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - The chicken embryo as an efficient model to test the function of muscle fusion genes in amniotes

AU - Sieiro, Daniel

AU - Véron, Nadège

AU - Marcelle, Christophe

PY - 2017/5/16

Y1 - 2017/5/16

N2 - The fusion of myoblasts into multinucleated myotubes is a crucial step of muscle growth during development and of muscle repair in the adult. While multiple genes were shown to play a role in this process, a vertebrate model where novel candidates can be tested and analyzed at high throughput and relative ease has been lacking. Here, we show that the early chicken embryo is a fast and robust model in which functional testing of muscle fusion candidate genes can be performed. We have used known modulators of muscle fusion, Rac1 and Cdc42, along with the in vivo electroporation of integrated, inducible vectors, to show that the chicken embryo is a suitable model in which their function can be tested and quantified. In addition to nuclei content, specific characteristics of the experimental model allow a fine characterization of additional morphological features that are nearly impossible to assess in other model organisms. This study should establish the chicken embryo as a cheap, reliable and powerful model in which novel vertebrate muscle fusion candidates can be evaluated.

AB - The fusion of myoblasts into multinucleated myotubes is a crucial step of muscle growth during development and of muscle repair in the adult. While multiple genes were shown to play a role in this process, a vertebrate model where novel candidates can be tested and analyzed at high throughput and relative ease has been lacking. Here, we show that the early chicken embryo is a fast and robust model in which functional testing of muscle fusion candidate genes can be performed. We have used known modulators of muscle fusion, Rac1 and Cdc42, along with the in vivo electroporation of integrated, inducible vectors, to show that the chicken embryo is a suitable model in which their function can be tested and quantified. In addition to nuclei content, specific characteristics of the experimental model allow a fine characterization of additional morphological features that are nearly impossible to assess in other model organisms. This study should establish the chicken embryo as a cheap, reliable and powerful model in which novel vertebrate muscle fusion candidates can be evaluated.

KW - cell fusion

KW - embryos

KW - muscle analysis

KW - somites

KW - muscle functions

KW - muscle fibers

KW - electroporation

KW - membrane fusion

UR - http://www.scopus.com/inward/record.url?scp=85019583602&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0177681

DO - 10.1371/journal.pone.0177681

M3 - Article

VL - 12

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 5

M1 - e0177681

ER -