THE CHARACTERISTICS OF [3H]‐CLONIDINE BINDING TO ANα‐ADRENOCEPTOR IN MEMBRANES FROM GUINEA‐PIG KIDNEY

B. JARROTT, W. J. LOUIS, R. J. SUMMERS

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Abstract

[3H]‐clonidine binds to membranes prepared from guinea‐pig kidney. At 25°C the binding is rapid and saturable. Scatchard analysis of the binding data showed that the Kd for [3H]‐clonidine binding in kidney membranes is 8.54 nM and the density of binding sites 12.5 pmol/g wet wt. tissue. Hill plots of the binding data showed that there were no cooperative site interactions associated with binding. [3H]‐clonidine binding could be displaced by drugs, the most potent being drugs with a high affinity for the α‐adrenoceptor. The neuroleptic drugs (+)‐butaclamol, ds‐clopenthixol and ds‐flupen‐thixol at high concentration also displaced [3H]‐clonidine binding. Drugs acting as agonists or antagonists of β‐adrenoceptors, histamine receptors, acetylcholine receptors as well as prostaglandins E1 E2, F and F, angiotensin II, arginine vasopressin, naloxone, nalorphine and pargyline had little effect on binding. It is likely that the binding site labelled by [3H]‐clonidine in guinea‐pig kidney membranes is an α‐adrenoceptor similar in some pharmacological aspects to an a2‐adrenoceptor. 1979 British Pharmacological Society

Original languageEnglish
Pages (from-to)663-670
Number of pages8
JournalBritish Journal of Pharmacology
Volume65
Issue number4
DOIs
Publication statusPublished - 1 Jan 1979

Cite this

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title = "THE CHARACTERISTICS OF [3H]‐CLONIDINE BINDING TO ANα‐ADRENOCEPTOR IN MEMBRANES FROM GUINEA‐PIG KIDNEY",
abstract = "[3H]‐clonidine binds to membranes prepared from guinea‐pig kidney. At 25°C the binding is rapid and saturable. Scatchard analysis of the binding data showed that the Kd for [3H]‐clonidine binding in kidney membranes is 8.54 nM and the density of binding sites 12.5 pmol/g wet wt. tissue. Hill plots of the binding data showed that there were no cooperative site interactions associated with binding. [3H]‐clonidine binding could be displaced by drugs, the most potent being drugs with a high affinity for the α‐adrenoceptor. The neuroleptic drugs (+)‐butaclamol, ds‐clopenthixol and ds‐flupen‐thixol at high concentration also displaced [3H]‐clonidine binding. Drugs acting as agonists or antagonists of β‐adrenoceptors, histamine receptors, acetylcholine receptors as well as prostaglandins E1 E2, Flα and F2α, angiotensin II, arginine vasopressin, naloxone, nalorphine and pargyline had little effect on binding. It is likely that the binding site labelled by [3H]‐clonidine in guinea‐pig kidney membranes is an α‐adrenoceptor similar in some pharmacological aspects to an a2‐adrenoceptor. 1979 British Pharmacological Society",
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THE CHARACTERISTICS OF [3H]‐CLONIDINE BINDING TO ANα‐ADRENOCEPTOR IN MEMBRANES FROM GUINEA‐PIG KIDNEY. / JARROTT, B.; LOUIS, W. J.; SUMMERS, R. J.

In: British Journal of Pharmacology, Vol. 65, No. 4, 01.01.1979, p. 663-670.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - THE CHARACTERISTICS OF [3H]‐CLONIDINE BINDING TO ANα‐ADRENOCEPTOR IN MEMBRANES FROM GUINEA‐PIG KIDNEY

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N2 - [3H]‐clonidine binds to membranes prepared from guinea‐pig kidney. At 25°C the binding is rapid and saturable. Scatchard analysis of the binding data showed that the Kd for [3H]‐clonidine binding in kidney membranes is 8.54 nM and the density of binding sites 12.5 pmol/g wet wt. tissue. Hill plots of the binding data showed that there were no cooperative site interactions associated with binding. [3H]‐clonidine binding could be displaced by drugs, the most potent being drugs with a high affinity for the α‐adrenoceptor. The neuroleptic drugs (+)‐butaclamol, ds‐clopenthixol and ds‐flupen‐thixol at high concentration also displaced [3H]‐clonidine binding. Drugs acting as agonists or antagonists of β‐adrenoceptors, histamine receptors, acetylcholine receptors as well as prostaglandins E1 E2, Flα and F2α, angiotensin II, arginine vasopressin, naloxone, nalorphine and pargyline had little effect on binding. It is likely that the binding site labelled by [3H]‐clonidine in guinea‐pig kidney membranes is an α‐adrenoceptor similar in some pharmacological aspects to an a2‐adrenoceptor. 1979 British Pharmacological Society

AB - [3H]‐clonidine binds to membranes prepared from guinea‐pig kidney. At 25°C the binding is rapid and saturable. Scatchard analysis of the binding data showed that the Kd for [3H]‐clonidine binding in kidney membranes is 8.54 nM and the density of binding sites 12.5 pmol/g wet wt. tissue. Hill plots of the binding data showed that there were no cooperative site interactions associated with binding. [3H]‐clonidine binding could be displaced by drugs, the most potent being drugs with a high affinity for the α‐adrenoceptor. The neuroleptic drugs (+)‐butaclamol, ds‐clopenthixol and ds‐flupen‐thixol at high concentration also displaced [3H]‐clonidine binding. Drugs acting as agonists or antagonists of β‐adrenoceptors, histamine receptors, acetylcholine receptors as well as prostaglandins E1 E2, Flα and F2α, angiotensin II, arginine vasopressin, naloxone, nalorphine and pargyline had little effect on binding. It is likely that the binding site labelled by [3H]‐clonidine in guinea‐pig kidney membranes is an α‐adrenoceptor similar in some pharmacological aspects to an a2‐adrenoceptor. 1979 British Pharmacological Society

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