The characteristics of adrenoceptors in homogenates of human cerebral cortex labelled by (3H)-rauwolscine

R. J. Summers, D. B. Barnett, S. R. Nahorski

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The alpha-2-adrenoceptor antagonist (3H)-rauwolscine has been used to label adrenoreptors in membranes from human cerebral cortex. The radioligand binds with high affinity (KD 2.08 nM) to a single population of sites with a density of 135 fmoles/mg protein. Adrenoceptor antagonists displaced binding in a simple monomolecular fashion with an order of affinity rauwolscine > yohimbine > phentolamine > corynanthine > prazosin indicating binding to alpha-2-adrenoceptors. Agonists displaced with an order of affinity clonidine > (-) adrenaline > (-) noradrenaline > dopamine > (-) isoprenaline but all displayed apparent Hill coefficients less than unity indicating heterogeneity of binding. The relatively high affinity of the alpha-1 antagonist prazosin for (3H)-rauwolscine binding sites in rat cerebral cortex was not observed in the human tissue which had pharmacological properties similar to those described previously in human platelet.

Original languageEnglish
Pages (from-to)1105-1112
Number of pages8
JournalLife Sciences
Issue number11
Publication statusPublished - 12 Sep 1983
Externally publishedYes

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