The centrosome-specific phosphorylation of Cnn by Polo/Plk1 drives Cnn scaffold assembly and centrosome maturation

Paul T. Conduit, Zhe Feng, Jennifer H. Richens, Janina Baumbach, Alan Wainman, Suruchi D. Bakshi, Jeroen Dobbelaere, Steven Johnson, Susan M. Lea, Jordan W. Raff

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96 Citations (Scopus)

Abstract

Centrosomes are important cell organizers. They consist of a pair of centrioles surrounded by pericentriolar material (PCM) that expands dramatically during mitosis-a process termed centrosome maturation. How centrosomes mature remains mysterious. Here, we identify a domain in Drosophila Cnn that appears to be phosphorylated by Polo/Plk1 specifically at centrosomes during mitosis. The phosphorylation promotes the assembly of a Cnn scaffold around the centrioles that is in constant flux, with Cnn molecules recruited continuously around the centrioles as the scaffold spreads slowly outward. Mutations that block Cnn phosphorylation strongly inhibit scaffold assembly and centrosome maturation, whereas phosphomimicking mutations allow Cnn to multimerize invitro and to spontaneously form cytoplasmic scaffolds invivo that organize microtubules independently of centrosomes. We conclude that Polo/Plk1 initiates the phosphorylation-dependent assembly of a Cnn scaffold around centrioles that is essential for efficient centrosome maturation in flies.

Original languageEnglish
Pages (from-to)659-669
Number of pages11
JournalDevelopmental Cell
Volume28
Issue number6
DOIs
Publication statusPublished - 31 Mar 2014
Externally publishedYes

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