THE CELL SURFACE PHENOTYPE OF MOUSE NEUTROPHILS

M. L. Hibbs, P. M. Hogarth, P. R. Collins, I. F.C. McKenzie

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Abstract

Using monoclonal antibodies, mouse peritoneal neutrophils were typed for the presence of 23 cell surface alloantigens, the expression of which was quantitated by flow cytofluorometry and compared with that of lymphocytes. The H‐2K and H‐2D alloantigens and β2‐microglobulin were present on all neutrophils, but Ia and Qa antigens were not detected. It was found that Ly‐5.1, Ly‐15.2, Ly‐21.2, Ly‐24.2 (Pgp‐1) and Ly‐25.1 were present on >90% of neutropils; Ly‐6.2 and Ly‐27.2 were absent, but Ly‐28.2 (encoded by an Ly‐6 linked gene), was present on >90% of neutrophils. As expected, the lymphocyte‐specific antigens Ly‐1.1, Ly‐2.2, Ly‐3.1, Ly‐7.2, Ly‐12.1 and Thy‐1.2 were absent from the neutrophils. When compared with lymphocytes, marked differences in alloantigen expression on neutrophils were seen for Ly‐5.1, Ly‐24.2 and Ly‐28.2. These studies should be of value in the study of neutrophil structure and function.

Original languageEnglish
Pages (from-to)247-257
Number of pages11
JournalInternational Journal of Immunogenetics
Volume12
Issue number4-5
DOIs
Publication statusPublished - 1 Jan 1985

Cite this

Hibbs, M. L. ; Hogarth, P. M. ; Collins, P. R. ; McKenzie, I. F.C. / THE CELL SURFACE PHENOTYPE OF MOUSE NEUTROPHILS. In: International Journal of Immunogenetics. 1985 ; Vol. 12, No. 4-5. pp. 247-257.
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THE CELL SURFACE PHENOTYPE OF MOUSE NEUTROPHILS. / Hibbs, M. L.; Hogarth, P. M.; Collins, P. R.; McKenzie, I. F.C.

In: International Journal of Immunogenetics, Vol. 12, No. 4-5, 01.01.1985, p. 247-257.

Research output: Contribution to journalArticleResearchpeer-review

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