The capsular polysaccharides of Pasteurella multocida serotypes B and E: Structural, genetic and serological comparisons

Frank St Michael; Chantelle M. Cairns; Perry Fleming; Evgeny V. Vinogradov; John D. Boyce; Marina Harper; Andrew D. Cox

doi:10.1093/glycob/cwaa069

The capsular polysaccharides of Pasteurella multocida serotypes B and E: Structural, genetic and serological comparisons

Frank St Michael, Chantelle M. Cairns, Perry Fleming, Evgeny V. Vinogradov, John D. Boyce, Marina Harper, Andrew D. Cox

Research output: Contribution to journal › Article › Research › peer-review

10 Citations (Scopus)

Abstract

We describe the structural characterization of the capsular polysaccharides (CPSs) of Pasteurella multocida serotypes B and E. CPS was isolated following organic solvent precipitation of the supernatant from flask grown cells. Structural analysis utilizing nuclear magnetic resonance spectroscopy enabled the determination of the CPS structures and revealed significant structural similarities between the two serotypes, but also provided an explanation for the serological distinction. This observation was extended by the development of polyclonal sera to the glycoconjugate of serotype B CPS that corroborated the structural likenesses and differences. Finally, identification of these structures enabled a more comprehensive interrogation of the genetic loci and prediction of roles for some of the encoded proteins in repeat unit biosynthesis.

Original language	English
Pages (from-to)	307-314
Number of pages	8
Journal	Glycobiology
Volume	31
Issue number	3
DOIs	https://doi.org/10.1093/glycob/cwaa069
Publication status	Published - Mar 2021

Keywords

capsular polysaccharides
NMR
Pasteurella multocida
serotyping
structure

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10.1093/glycob/cwaa069

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title = "The capsular polysaccharides of Pasteurella multocida serotypes B and E: Structural, genetic and serological comparisons",

abstract = "We describe the structural characterization of the capsular polysaccharides (CPSs) of Pasteurella multocida serotypes B and E. CPS was isolated following organic solvent precipitation of the supernatant from flask grown cells. Structural analysis utilizing nuclear magnetic resonance spectroscopy enabled the determination of the CPS structures and revealed significant structural similarities between the two serotypes, but also provided an explanation for the serological distinction. This observation was extended by the development of polyclonal sera to the glycoconjugate of serotype B CPS that corroborated the structural likenesses and differences. Finally, identification of these structures enabled a more comprehensive interrogation of the genetic loci and prediction of roles for some of the encoded proteins in repeat unit biosynthesis. ",

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doi = "10.1093/glycob/cwaa069",

language = "English",

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AU - Michael, Frank St

AU - Cairns, Chantelle M.

AU - Fleming, Perry

AU - Vinogradov, Evgeny V.

AU - Boyce, John D.

AU - Harper, Marina

AU - Cox, Andrew D.

N1 - Publisher Copyright: © 2020 The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: [email protected]. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/3

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N2 - We describe the structural characterization of the capsular polysaccharides (CPSs) of Pasteurella multocida serotypes B and E. CPS was isolated following organic solvent precipitation of the supernatant from flask grown cells. Structural analysis utilizing nuclear magnetic resonance spectroscopy enabled the determination of the CPS structures and revealed significant structural similarities between the two serotypes, but also provided an explanation for the serological distinction. This observation was extended by the development of polyclonal sera to the glycoconjugate of serotype B CPS that corroborated the structural likenesses and differences. Finally, identification of these structures enabled a more comprehensive interrogation of the genetic loci and prediction of roles for some of the encoded proteins in repeat unit biosynthesis.

AB - We describe the structural characterization of the capsular polysaccharides (CPSs) of Pasteurella multocida serotypes B and E. CPS was isolated following organic solvent precipitation of the supernatant from flask grown cells. Structural analysis utilizing nuclear magnetic resonance spectroscopy enabled the determination of the CPS structures and revealed significant structural similarities between the two serotypes, but also provided an explanation for the serological distinction. This observation was extended by the development of polyclonal sera to the glycoconjugate of serotype B CPS that corroborated the structural likenesses and differences. Finally, identification of these structures enabled a more comprehensive interrogation of the genetic loci and prediction of roles for some of the encoded proteins in repeat unit biosynthesis.

KW - capsular polysaccharides

KW - NMR

KW - Pasteurella multocida

KW - serotyping

KW - structure

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JO - Glycobiology

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ER -

The capsular polysaccharides of Pasteurella multocida serotypes B and E: Structural, genetic and serological comparisons

Abstract

Keywords

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