The avian embryo as a model system for skeletal myogenesis

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

Abstract

This review will focus on the use of the chicken and quail as model systems to analyze myogenesis and as such will emphasize the experimental approaches that are strongest in these systems-the amenability of the avian embryo to manipulation and in ovo observation. During somite differentiation, a wide spectrum of developmental processes occur such as cellular differentiation, migration, and fusion. Cell lineage studies combined with recent advancements in cell imaging allow these biological phenomena to be readily observed and hypotheses tested extremely rapidly-a strength that is restricted to the avian system. A clear weakness of the chicken in the past has been genetic approaches to modulate gene function. Recent advances in the electroporation of expression vectors, siRNA constructs, and use of tissue specific reporters have opened the door to increasingly sophisticated experiments that address questions of interest not only to the somite/muscle field in particular but also fundamental to biology in general. Importantly, an ever-growing body of evidence indicates that somite differentiation in birds is indistinguishable to that of mammals; therefore, these avian studies complement the complex genetic models of the mouse.
Original languageEnglish
Title of host publicationVertebrate Myogenesis
Subtitle of host publicationStem Cells and Precursors
EditorsBeate Brand-Saberi
Place of PublicationBerlin Germany
PublisherSpringer
Pages99-122
Number of pages24
ISBN (Print)9783662446072
DOIs
Publication statusPublished - 2015

Publication series

NameResults and Problems in Cell Differentiation
PublisherSpringer
Volume56

Cite this

Hirst, C. E., & Marcelle, C. (2015). The avian embryo as a model system for skeletal myogenesis. In B. Brand-Saberi (Ed.), Vertebrate Myogenesis: Stem Cells and Precursors (pp. 99-122). (Results and Problems in Cell Differentiation; Vol. 56). Berlin Germany: Springer. https://doi.org/10.1007/978-3-662-44608-9_5
Hirst, Claire E ; Marcelle, Christophe. / The avian embryo as a model system for skeletal myogenesis. Vertebrate Myogenesis: Stem Cells and Precursors. editor / Beate Brand-Saberi. Berlin Germany : Springer, 2015. pp. 99-122 (Results and Problems in Cell Differentiation).
@inbook{778dbc2cc269440e9d755cf477c14d89,
title = "The avian embryo as a model system for skeletal myogenesis",
abstract = "This review will focus on the use of the chicken and quail as model systems to analyze myogenesis and as such will emphasize the experimental approaches that are strongest in these systems-the amenability of the avian embryo to manipulation and in ovo observation. During somite differentiation, a wide spectrum of developmental processes occur such as cellular differentiation, migration, and fusion. Cell lineage studies combined with recent advancements in cell imaging allow these biological phenomena to be readily observed and hypotheses tested extremely rapidly-a strength that is restricted to the avian system. A clear weakness of the chicken in the past has been genetic approaches to modulate gene function. Recent advances in the electroporation of expression vectors, siRNA constructs, and use of tissue specific reporters have opened the door to increasingly sophisticated experiments that address questions of interest not only to the somite/muscle field in particular but also fundamental to biology in general. Importantly, an ever-growing body of evidence indicates that somite differentiation in birds is indistinguishable to that of mammals; therefore, these avian studies complement the complex genetic models of the mouse.",
author = "Hirst, {Claire E} and Christophe Marcelle",
year = "2015",
doi = "10.1007/978-3-662-44608-9_5",
language = "English",
isbn = "9783662446072",
series = "Results and Problems in Cell Differentiation",
publisher = "Springer",
pages = "99--122",
editor = "Beate Brand-Saberi",
booktitle = "Vertebrate Myogenesis",

}

Hirst, CE & Marcelle, C 2015, The avian embryo as a model system for skeletal myogenesis. in B Brand-Saberi (ed.), Vertebrate Myogenesis: Stem Cells and Precursors. Results and Problems in Cell Differentiation, vol. 56, Springer, Berlin Germany, pp. 99-122. https://doi.org/10.1007/978-3-662-44608-9_5

The avian embryo as a model system for skeletal myogenesis. / Hirst, Claire E; Marcelle, Christophe.

Vertebrate Myogenesis: Stem Cells and Precursors. ed. / Beate Brand-Saberi. Berlin Germany : Springer, 2015. p. 99-122 (Results and Problems in Cell Differentiation; Vol. 56).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

TY - CHAP

T1 - The avian embryo as a model system for skeletal myogenesis

AU - Hirst, Claire E

AU - Marcelle, Christophe

PY - 2015

Y1 - 2015

N2 - This review will focus on the use of the chicken and quail as model systems to analyze myogenesis and as such will emphasize the experimental approaches that are strongest in these systems-the amenability of the avian embryo to manipulation and in ovo observation. During somite differentiation, a wide spectrum of developmental processes occur such as cellular differentiation, migration, and fusion. Cell lineage studies combined with recent advancements in cell imaging allow these biological phenomena to be readily observed and hypotheses tested extremely rapidly-a strength that is restricted to the avian system. A clear weakness of the chicken in the past has been genetic approaches to modulate gene function. Recent advances in the electroporation of expression vectors, siRNA constructs, and use of tissue specific reporters have opened the door to increasingly sophisticated experiments that address questions of interest not only to the somite/muscle field in particular but also fundamental to biology in general. Importantly, an ever-growing body of evidence indicates that somite differentiation in birds is indistinguishable to that of mammals; therefore, these avian studies complement the complex genetic models of the mouse.

AB - This review will focus on the use of the chicken and quail as model systems to analyze myogenesis and as such will emphasize the experimental approaches that are strongest in these systems-the amenability of the avian embryo to manipulation and in ovo observation. During somite differentiation, a wide spectrum of developmental processes occur such as cellular differentiation, migration, and fusion. Cell lineage studies combined with recent advancements in cell imaging allow these biological phenomena to be readily observed and hypotheses tested extremely rapidly-a strength that is restricted to the avian system. A clear weakness of the chicken in the past has been genetic approaches to modulate gene function. Recent advances in the electroporation of expression vectors, siRNA constructs, and use of tissue specific reporters have opened the door to increasingly sophisticated experiments that address questions of interest not only to the somite/muscle field in particular but also fundamental to biology in general. Importantly, an ever-growing body of evidence indicates that somite differentiation in birds is indistinguishable to that of mammals; therefore, these avian studies complement the complex genetic models of the mouse.

UR - http://www.ncbi.nlm.nih.gov/pubmed/25344668

U2 - 10.1007/978-3-662-44608-9_5

DO - 10.1007/978-3-662-44608-9_5

M3 - Chapter (Book)

SN - 9783662446072

T3 - Results and Problems in Cell Differentiation

SP - 99

EP - 122

BT - Vertebrate Myogenesis

A2 - Brand-Saberi, Beate

PB - Springer

CY - Berlin Germany

ER -

Hirst CE, Marcelle C. The avian embryo as a model system for skeletal myogenesis. In Brand-Saberi B, editor, Vertebrate Myogenesis: Stem Cells and Precursors. Berlin Germany: Springer. 2015. p. 99-122. (Results and Problems in Cell Differentiation). https://doi.org/10.1007/978-3-662-44608-9_5