The autoradiographic localization of adenylate cyclase in rat kidney using [3H]forskolin

Lynne R. McMartin; Roger J. Summers

doi:10.1016/0006-2952(90)90280-X

The autoradiographic localization of adenylate cyclase in rat kidney using [3H]forskolin

Lynne R. McMartin, Roger J. Summers

Drug Discovery Biology

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Abstract

The localization of [³H]forskolin binding to microscope slide mounted sections of rat kidney has been examined using autoradiography. Saturation studies showed [³H]forskolin binding to two sites, a high affinity site (K_D = 8.7 nM, B_max = 0.14 pmol/mg protein) and a low affinity site (K_D = 6.7 μM, B_max = 11.0 pmol/mg protein). Autoradiographs showed high affinity binding (thought to identify stimulatory guanine nucleotide binding protein (Gs)-linked adenylate cyclase) to all renal structures known to possess hormone sensitive adenylate cyclase, including all tubular segments, glomeruli and blood vessels. High concentrations of binding were associated with a portion of the proximal tubule and with papillary collecting tubules and ducts.

Original language	English
Pages (from-to)	1019-1028
Number of pages	10
Journal	Biochemical Pharmacology
Volume	39
Issue number	6
DOIs	https://doi.org/10.1016/0006-2952(90)90280-X
Publication status	Published - 15 Mar 1990

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title = "The autoradiographic localization of adenylate cyclase in rat kidney using [3H]forskolin",

abstract = "The localization of [3H]forskolin binding to microscope slide mounted sections of rat kidney has been examined using autoradiography. Saturation studies showed [3H]forskolin binding to two sites, a high affinity site (KD = 8.7 nM, Bmax = 0.14 pmol/mg protein) and a low affinity site (KD = 6.7 μM, Bmax = 11.0 pmol/mg protein). Autoradiographs showed high affinity binding (thought to identify stimulatory guanine nucleotide binding protein (Gs)-linked adenylate cyclase) to all renal structures known to possess hormone sensitive adenylate cyclase, including all tubular segments, glomeruli and blood vessels. High concentrations of binding were associated with a portion of the proximal tubule and with papillary collecting tubules and ducts.",

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AU - Summers, Roger J.

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N2 - The localization of [3H]forskolin binding to microscope slide mounted sections of rat kidney has been examined using autoradiography. Saturation studies showed [3H]forskolin binding to two sites, a high affinity site (KD = 8.7 nM, Bmax = 0.14 pmol/mg protein) and a low affinity site (KD = 6.7 μM, Bmax = 11.0 pmol/mg protein). Autoradiographs showed high affinity binding (thought to identify stimulatory guanine nucleotide binding protein (Gs)-linked adenylate cyclase) to all renal structures known to possess hormone sensitive adenylate cyclase, including all tubular segments, glomeruli and blood vessels. High concentrations of binding were associated with a portion of the proximal tubule and with papillary collecting tubules and ducts.

AB - The localization of [3H]forskolin binding to microscope slide mounted sections of rat kidney has been examined using autoradiography. Saturation studies showed [3H]forskolin binding to two sites, a high affinity site (KD = 8.7 nM, Bmax = 0.14 pmol/mg protein) and a low affinity site (KD = 6.7 μM, Bmax = 11.0 pmol/mg protein). Autoradiographs showed high affinity binding (thought to identify stimulatory guanine nucleotide binding protein (Gs)-linked adenylate cyclase) to all renal structures known to possess hormone sensitive adenylate cyclase, including all tubular segments, glomeruli and blood vessels. High concentrations of binding were associated with a portion of the proximal tubule and with papillary collecting tubules and ducts.

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The autoradiographic localization of adenylate cyclase in rat kidney using [3H]forskolin

Abstract

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