The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard

Lifeng Peng; Eugene A Kapp; David Fenyo; Min-Seok Kwon; Pu Jiang; Songfeng Wu; Ying Jiang; Marie Isabel Aguilar; Nikhat Ahmed; Mark S Baker; Zongwei Cai; Yu-Ju Chen; Phan Van Chi; Maxey CM Chung; Fuchu He; Alice CL Len; Pao-Chi Liao; Kazuyuki Nakamura; Sai Ming Ngai; Young-Ki Paik; Tai-Long Pan; Terence CW Poon; Ghasem H Salekdeh; Richard J Simpson; Ravi Sirdeshmukh; Chantragan Srisomsap; Jisnuson Svasti; Yu-Chang Tyan; Florian S Dreyer; Danyl McLauchlan; Pisana Rawson; T William Jordan

doi:10.1002/pmic.201000126

The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard

Lifeng Peng, Eugene A Kapp, David Fenyo, Min-Seok Kwon, Pu Jiang, Songfeng Wu, Ying Jiang, Marie Isabel Aguilar, Nikhat Ahmed, Mark S Baker, Zongwei Cai, Yu-Ju Chen, Phan Van Chi, Maxey CM Chung, Fuchu He, Alice CL Len, Pao-Chi Liao, Kazuyuki Nakamura, Sai Ming Ngai, Young-Ki PaikTai-Long Pan, Terence CW Poon, Ghasem H Salekdeh, Richard J Simpson, Ravi Sirdeshmukh, Chantragan Srisomsap, Jisnuson Svasti, Yu-Chang Tyan, Florian S Dreyer, Danyl McLauchlan, Pisana Rawson, T William Jordan

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

16 Citations (Scopus)

Abstract

The Asia Oceania Human Proteome Organisation has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multi-laboratory project is based on analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. Here we present the strategy used for preparation and initial characterisation of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of seventeen independent datasets from five types of proteomic workflows is in progress.

Original language	English
Pages (from-to)	4142 - 4148
Number of pages	7
Journal	Proteomics
Volume	10
Issue number	22
DOIs	https://doi.org/10.1002/pmic.201000126
Publication status	Published - 2010

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Peng, L., Kapp, E. A., Fenyo, D., Kwon, M.-S., Jiang, P., Wu, S., Jiang, Y., Aguilar, M. I., Ahmed, N., Baker, M. S., Cai, Z., Chen, Y.-J., Chi, P. V., Chung, M. CM., He, F., Len, A. CL., Liao, P.-C., Nakamura, K., Ngai, S. M., ... Jordan, T. W. (2010). The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard. Proteomics, 10(22), 4142 - 4148. https://doi.org/10.1002/pmic.201000126

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title = "The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard",

abstract = "The Asia Oceania Human Proteome Organisation has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multi-laboratory project is based on analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. Here we present the strategy used for preparation and initial characterisation of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of seventeen independent datasets from five types of proteomic workflows is in progress.",

author = "Lifeng Peng and Kapp, \{Eugene A\} and David Fenyo and Min-Seok Kwon and Pu Jiang and Songfeng Wu and Ying Jiang and Aguilar, \{Marie Isabel\} and Nikhat Ahmed and Baker, \{Mark S\} and Zongwei Cai and Yu-Ju Chen and Chi, \{Phan Van\} and Chung, \{Maxey CM\} and Fuchu He and Len, \{Alice CL\} and Pao-Chi Liao and Kazuyuki Nakamura and Ngai, \{Sai Ming\} and Young-Ki Paik and Tai-Long Pan and Poon, \{Terence CW\} and Salekdeh, \{Ghasem H\} and Simpson, \{Richard J\} and Ravi Sirdeshmukh and Chantragan Srisomsap and Jisnuson Svasti and Yu-Chang Tyan and Dreyer, \{Florian S\} and Danyl McLauchlan and Pisana Rawson and Jordan, \{T William\}",

year = "2010",

doi = "10.1002/pmic.201000126",

language = "English",

volume = "10",

pages = "4142 -- 4148",

journal = "Proteomics",

issn = "1615-9853",

publisher = "Wiley-VCH Verlag GmbH \& Co. KGaA",

number = "22",

}

Peng, L, Kapp, EA, Fenyo, D, Kwon, M-S, Jiang, P, Wu, S, Jiang, Y, Aguilar, MI, Ahmed, N, Baker, MS, Cai, Z, Chen, Y-J, Chi, PV, Chung, MCM, He, F, Len, ACL, Liao, P-C, Nakamura, K, Ngai, SM, Paik, Y-K, Pan, T-L, Poon, TCW, Salekdeh, GH, Simpson, RJ, Sirdeshmukh, R, Srisomsap, C, Svasti, J, Tyan, Y-C, Dreyer, FS, McLauchlan, D, Rawson, P & Jordan, TW 2010, 'The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard', Proteomics, vol. 10, no. 22, pp. 4142 - 4148. https://doi.org/10.1002/pmic.201000126

TY - JOUR

T1 - The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard

AU - Peng, Lifeng

AU - Kapp, Eugene A

AU - Fenyo, David

AU - Kwon, Min-Seok

AU - Jiang, Pu

AU - Wu, Songfeng

AU - Jiang, Ying

AU - Aguilar, Marie Isabel

AU - Ahmed, Nikhat

AU - Baker, Mark S

AU - Cai, Zongwei

AU - Chen, Yu-Ju

AU - Chi, Phan Van

AU - Chung, Maxey CM

AU - He, Fuchu

AU - Len, Alice CL

AU - Liao, Pao-Chi

AU - Nakamura, Kazuyuki

AU - Ngai, Sai Ming

AU - Paik, Young-Ki

AU - Pan, Tai-Long

AU - Poon, Terence CW

AU - Salekdeh, Ghasem H

AU - Simpson, Richard J

AU - Sirdeshmukh, Ravi

AU - Srisomsap, Chantragan

AU - Svasti, Jisnuson

AU - Tyan, Yu-Chang

AU - Dreyer, Florian S

AU - McLauchlan, Danyl

AU - Rawson, Pisana

AU - Jordan, T William

PY - 2010

Y1 - 2010

N2 - The Asia Oceania Human Proteome Organisation has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multi-laboratory project is based on analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. Here we present the strategy used for preparation and initial characterisation of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of seventeen independent datasets from five types of proteomic workflows is in progress.

AB - The Asia Oceania Human Proteome Organisation has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multi-laboratory project is based on analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. Here we present the strategy used for preparation and initial characterisation of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of seventeen independent datasets from five types of proteomic workflows is in progress.

UR - http://onlinelibrary.wiley.com/doi/10.1002/pmic.201000126/pdf

UR - https://www.scopus.com/pages/publications/78449297707

U2 - 10.1002/pmic.201000126

DO - 10.1002/pmic.201000126

M3 - Article

SN - 1615-9853

VL - 10

SP - 4142

EP - 4148

JO - Proteomics

JF - Proteomics

IS - 22

ER -

The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard

Abstract

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