The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard

Lifeng Peng, Eugene A Kapp, David Fenyo, Min-Seok Kwon, Pu Jiang, Songfeng Wu, Ying Jiang, Marie Isabel Aguilar, Nikhat Ahmed, Mark S Baker, Zongwei Cai, Yu-Ju Chen, Phan Van Chi, Maxey CM Chung, Fuchu He, Alice CL Len, Pao-Chi Liao, Kazuyuki Nakamura, Sai Ming Ngai, Young-Ki PaikTai-Long Pan, Terence CW Poon, Ghasem H Salekdeh, Richard J Simpson, Ravi Sirdeshmukh, Chantragan Srisomsap, Jisnuson Svasti, Yu-Chang Tyan, Florian S Dreyer, Danyl McLauchlan, Pisana Rawson, T William Jordan

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Abstract

The Asia Oceania Human Proteome Organisation has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multi-laboratory project is based on analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. Here we present the strategy used for preparation and initial characterisation of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of seventeen independent datasets from five types of proteomic workflows is in progress.
Original languageEnglish
Pages (from-to)4142 - 4148
Number of pages7
JournalProteomics
Volume10
Issue number22
DOIs
Publication statusPublished - 2010

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