The application of high throughput siRNA screening technology to study host-pathogen interactions

Li Ou, Dan Duan, Jinhua Wu, Edouard C Nice, Canhua Huang

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)


Recent advances in high-throughput screening technologies have accelerated the identification and characterization of potential factors involved in host-virus interactions, facilitating early detection and diagnosis of diseases, as well as providing promising drug targets. The last decade has seen a plethora of successful examples of high-throughput screening approaches, especially siRNA screening. With support from protein interaction studies, mRNA expression profiling, and bioinformatics, siRNA screening has also been successfully utilized to identify host factors required for a number of viruses including HIV, West Nile virus and H1N1 virus. Such studies have raised the awareness of virologists, and have opened a new chapter of global analysis of host-pathogen interactions. However, to play a more defining role in prognostics, diagnostics and therapeutics for virus diseases, acknowledged drawbacks, including false positives and negatives, inherent in this technology, must be successfully addressed.
Original languageEnglish
Pages (from-to)299 - 305
Number of pages7
JournalCombinatorial Chemistry and High Throughput Screening
Issue number4
Publication statusPublished - 2012

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