The antibacterial effect of g3-poly-amidoamine dendrimer on gram negative and gram positive bacteria in aqueous solutions

Ali Saifoury Mofrad, Mohammad Javad Mohammadi, Hossein Jafari Mansoorian, Yusef Omidi Khaniabadid, Mohammad Ahmadi Jebeli, Narges Khanjani, Mohammad Khoshgoftar, Ahmad Reza Yari

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1 Citation (Scopus)

Abstract

Dendrimers are symmetric, round and ramose macromolecules made up of monomers which have a certain structural order. The aim of this study was to evaluate the antibacterial effects of different concentrations of G3-poly-amidoamine dendrimer (G3-PAD) on 7 species of gram-negative and gram-positive bacteria in aqueous solutions. Different concentrations of G3-PAD were prepared and from each bacterial species, a suspension with a different pH was prepared according to the McFarland 0.5 standard. Bacteria were left in a 37°C incubator and shaker from 0 to 60 min, and were cultivated on a Mueller-Hinton Agar plate. The bacteria that grew on each plate were counted and analyzed. The results showed that the antibacterial effect of G3-PAD in aqueous solutions was directly related to dendrimer concentration, contact time and pH. The strongest effect on Salmonella and Bacillus Subtilis was at pH = 6.5 and 7.5 and contact time 45 min, and in pH = 9, contact time 30 min; for Enterococcus faecalis in all three pH, it was 60 min; for Staphylococcus Aureus in pH = 6.5 and 7.5, 60 min and in pH = 9, 30 min; for E. coli in pH = 6.5 and 7.5, 60 min and in pH = 9, 45 min. G3-PAD showed its strongest effect on Shigella bacteria in pH = 6.5, contact time 60 min. In pH = 7.5 there was no optimum condition and in pH = 9, the optimum contact time was 60 min. The strongest effect of G3-PAD on all species was seen in 2000 ppm concentration. These results show a new strategy to improve bacteria elimination from aqueous solutions. However, safely using dendrimers for disinfecting drinking water still needs more research.

Original languageEnglish
Pages (from-to)223-231
Number of pages9
JournalDesalination and Water Treatment
Volume124
DOIs
Publication statusPublished - 1 Aug 2018
Externally publishedYes

Keywords

  • Aqueous solutions
  • G3-poly-amidoamine dendrimer
  • Gram negative bacteria
  • Gram-positive bacteria
  • Polymer

Cite this

Mofrad, A. S., Mohammadi, M. J., Mansoorian, H. J., Khaniabadid, Y. O., Jebeli, M. A., Khanjani, N., Khoshgoftar, M., & Yari, A. R. (2018). The antibacterial effect of g3-poly-amidoamine dendrimer on gram negative and gram positive bacteria in aqueous solutions. Desalination and Water Treatment, 124, 223-231. https://doi.org/10.5004/dwt.2018.22436