The ADAMTS5 metzincin regulates zebrafish somite differentiation

Carolyn M. Dancevic, Yann Gibert, Joachim Berger, Adam D. Smith, Clifford Liongue, Nicole Stupka, Alister C. Ward, Daniel R. McCulloch

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The ADAMTS5 metzincin, a secreted zinc-dependent metalloproteinase, modulates the extracellular matrix (ECM) during limb morphogenesis and other developmental processes. Here, the role of ADAMTS5 was investigated by knockdown of zebrafish adamts5 during embryogenesis. This revealed impaired Sonic Hedgehog (Shh) signaling during somite patterning and early myogenesis. Notably, synergistic regulation of myod expression by ADAMTS5 and Shh during somite differentiation was observed. These roles were not dependent upon the catalytic activity of ADAMTS5. These data identify a non-enzymatic function for ADAMTS5 in regulating an important cell signaling pathway that impacts on muscle development, with implications for musculoskeletal diseases in which ADAMTS5 and Shh have been associated.

Original languageEnglish
Article number766
Number of pages12
JournalInternational Journal of Molecular Sciences
Issue number3
Publication statusPublished - 7 Mar 2018


  • Extracellular matrix
  • Metalloproteinase
  • Muscle
  • Somite
  • Zebrafish

Cite this

Dancevic, C. M., Gibert, Y., Berger, J., Smith, A. D., Liongue, C., Stupka, N., Ward, A. C., & McCulloch, D. R. (2018). The ADAMTS5 metzincin regulates zebrafish somite differentiation. International Journal of Molecular Sciences, 19(3), [766].