The β-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates

Hyeryun Choe, Michael Farzan, Ying Sun, Nancy Sullivan, Barrett Rollins, Paul D. Ponath, Lijun Wu, Charles R. Mackay, Gregory LaRosa, Walter Newman, Norma Gerard, Craig Gerard, Joseph Sodroski

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2047 Citations (Scopus)

Abstract

We examined the ability of chemokine receptors and related G protein- coupled receptors to facilitate infection by primary, clinical HIV-1 isolates. CCR5, when expressed along with CD4, the HIV-1 receptor, allowed cell lines resistant to most primary HIV-1 isolates to be infected. CCR3 facilitated infection by a more restricted subset of primary viruses, and binding of the CCR3 ligand, eotaxin, inhibited infection by these isolates. Utilization of CCR3 and CCR5 on the target cell depended upon the sequence of the third variable (V3) region of the HIV-1 gp120 exterior envelope glycoprotein. The ability of various members of the chemokine receptor family to support the early stages of HIV-1 infection helps to explain viral tropism and β-chemokine inhibition of primary HIV-1 isolates.

Original languageEnglish
Pages (from-to)1135-1148
Number of pages14
JournalCell
Volume85
Issue number7
DOIs
Publication statusPublished - 28 Jun 1996
Externally publishedYes

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