@article{bf842c5c3d3e4907b93d97713dc80f05,
title = "Tex19.1 inhibits the N-end rule pathway and maintains acetylated SMC3 cohesin and sister chromatid cohesion in oocytes",
abstract = "Age-dependent oocyte aneuploidy, a major cause of Down syndrome, is associated with declining sister chromatid cohesion in postnatal oocytes. Here we show that cohesion in postnatal mouse oocytes is regulated by Tex19.1. We show Tex19.1−/− oocytes have defects maintaining chiasmata, missegregate their chromosomes during meiosis, and transmit aneuploidies to the next generation. Furthermore, we show that mouse Tex19.1 inhibits N-end rule protein degradation mediated by its interacting partner UBR2, and that Ubr2 itself has a previously undescribed role in negatively regulating the acetylated SMC3 subpopulation of cohesin in mitotic somatic cells. Lastly, we show that acetylated SMC3 is associated with meiotic chromosome axes in mouse oocytes, and that this population of cohesin is specifically depleted in the absence of Tex19.1. These findings indicate that Tex19.1 regulates UBR protein activity to maintain acetylated SMC3 and sister chromatid cohesion in postnatal oocytes and prevent aneuploidy from arising in the female germline.",
author = "Judith Reichmann and Karen Dobie and Lister, {Lisa M.} and Crichton, {James H.} and Diana Best and Marie MacLennan and David Read and Raymond, {Eleanor S.} and Hung, {Chao Chun} and Shelagh Boyle and Katsuhiko Shirahige and Cooke, {Howard J.} and Mary Herbert and Adams, {Ian R.}",
note = "Funding Information: Funding: J. Reichmann and E.S. Raymond were supported by Medical Research Council Doctoral Training Awards. K. Dobie, J.H. Crichton, D. Best, M. MacLennan, D. Read, C.-C. Hung, S. Boyle, H.J. Cooke, and I.R. Adams were supported by Medical Research Council Intramural Program Grants MC_PC_U127580973 and MC_UU_00007/6. K. Shirahige was supported by Japan Science and Technology Agency CREST grant JPMJCR18S5. L.M. Lister and M. Herbert were supported by funding from the European Union{\textquoteright}s Horizon 2020 - Research and Innovation Framework Programme under grant agreement no. 634113 (GermAge) and funding from the Medical Research Council (MR/J003603/1). The authors declare no competing financial interests. Funding Information: We thank Christer H??g and Nico Dantuma (Karolinska Institutet, Stockholm, Sweden) for providing anti-REC8 antibodies and Ub-L-GFP constructs, respectively. We are grateful to Sally Shirran and Catherine Botting (Mass Spectrometry and Proteomics Facility, University of St. Andrews, St. Andrews, UK) for performing mass spectrometry on anti-YFP immunoprecipitates; to imaging services at Medical Research Council Human Genetics Unit; and to bioresearch and veterinary services at University of Edinburgh. We thank Wendy Bickmore and Nick Hastie (both Medical Research Council Human Genetics Unit), Jan Ellenberg (European Molecular Biology Laboratory, Heidelberg, Germany), and Mariana Coelho Correia Da Silva (Research Institute of Molecular Pathology, Vienna, Austria) for support, advice, and helpful suggestions, and Javier Caceres (Medical Research Council Human Genetics Unit) for comments on the manuscript. Funding: J. Reichmann and E.S. Raymond were supported by Medical Research Council Doctoral Training Awards. K. Dobie, J.H. Crichton, D. Best, M. MacLennan, D. Read, C.-C. Hung, S. Boyle, H.J. Cooke, and I.R. Adams were supported by Medical Research Council Intramural Program Grants MC_PC_U127580973 and MC_UU_00007/6. K. Shirahige was supported by Japan Science and Technology Agency CREST grant JPMJCR18S5. L.M. Lister and M. Herbert were supported by funding from the European Union?s Horizon 2020 - Research and Innovation Framework Programme under grant agreement no. 634113 (GermAge) and funding from the Medical Research Council (MR/J003603/1). Publisher Copyright: {\textcopyright} 2020 Reichmann et al.",
year = "2020",
month = may,
day = "4",
doi = "10.1083/jcb.201702123",
language = "English",
volume = "219",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "The Rockefeller University Press",
number = "5",
}