Tetraspanin microdomains control localized protein kinase C signaling in B cells

Malou Zuidscherwoude, Vera Marie E. Dunlock, Geert Van Den Bogaart, Sjoerd J. Van Deventer, Alie van der Schaaf, Jenny Van Oostrum, Joachim Goedhart, Joanna In't Hout, Günter J. Hämmerling, Satoshi Tanaka, André Nadler, Carsten Schultz, Mark D. Wright, Merel J.W. Adjobo-Hermans, Annemiek B. Van Spriel

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Abstract

Activation of B cells by the binding of antigens to the B cell receptor (BCR) requires the protein kinase C (PKC) family member PKCβ. Because PKCs must translocate to the plasma membrane to become activated, we investigated the mechanisms regulating their spatial distribution in mouse and human B cells. Through live-cell imaging, we showed that BCR-stimulated production of the second messenger diacylglycerol (DAG) resulted in the translocation of PKCβ from the cytosol to plasma membrane regions containing the tetraspanin protein CD53. CD53 was specifically enriched at sites of BCR signaling, suggesting that BCR-dependent PKC signaling was initiated at these tetraspanin microdomains. Fluorescence lifetime imaging microscopy studies confirmed the molecular recruitment of PKC to CD53-containing microdomains, which required the amino terminus of CD53. Furthermore, we showed that Cd53-deficient B cells were defective in the phosphorylation of PKC substrates. Consistent with this finding, PKC recruitment to the plasma membrane was impaired in both mouse and human CD53-deficient B cells compared to that in their wild-type counterparts. These data suggest that CD53 promotes BCR-dependent PKC signaling by recruiting PKC to the plasma membrane so that it can phosphorylate its substrates and that tetraspanin-containing microdomains can act as signaling hotspots in the plasma membrane. 2017

Original languageEnglish
Article numberaag2755
Number of pages15
JournalScience Signaling
Volume10
Issue number478
DOIs
Publication statusPublished - 9 May 2017

Cite this

Zuidscherwoude, M., Dunlock, V. M. E., Van Den Bogaart, G., Van Deventer, S. J., van der Schaaf, A., Van Oostrum, J., ... Van Spriel, A. B. (2017). Tetraspanin microdomains control localized protein kinase C signaling in B cells. Science Signaling, 10(478), [aag2755]. https://doi.org/10.1126/scisignal.aag2755
Zuidscherwoude, Malou ; Dunlock, Vera Marie E. ; Van Den Bogaart, Geert ; Van Deventer, Sjoerd J. ; van der Schaaf, Alie ; Van Oostrum, Jenny ; Goedhart, Joachim ; In't Hout, Joanna ; Hämmerling, Günter J. ; Tanaka, Satoshi ; Nadler, André ; Schultz, Carsten ; Wright, Mark D. ; Adjobo-Hermans, Merel J.W. ; Van Spriel, Annemiek B. / Tetraspanin microdomains control localized protein kinase C signaling in B cells. In: Science Signaling. 2017 ; Vol. 10, No. 478.
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title = "Tetraspanin microdomains control localized protein kinase C signaling in B cells",
abstract = "Activation of B cells by the binding of antigens to the B cell receptor (BCR) requires the protein kinase C (PKC) family member PKCβ. Because PKCs must translocate to the plasma membrane to become activated, we investigated the mechanisms regulating their spatial distribution in mouse and human B cells. Through live-cell imaging, we showed that BCR-stimulated production of the second messenger diacylglycerol (DAG) resulted in the translocation of PKCβ from the cytosol to plasma membrane regions containing the tetraspanin protein CD53. CD53 was specifically enriched at sites of BCR signaling, suggesting that BCR-dependent PKC signaling was initiated at these tetraspanin microdomains. Fluorescence lifetime imaging microscopy studies confirmed the molecular recruitment of PKC to CD53-containing microdomains, which required the amino terminus of CD53. Furthermore, we showed that Cd53-deficient B cells were defective in the phosphorylation of PKC substrates. Consistent with this finding, PKC recruitment to the plasma membrane was impaired in both mouse and human CD53-deficient B cells compared to that in their wild-type counterparts. These data suggest that CD53 promotes BCR-dependent PKC signaling by recruiting PKC to the plasma membrane so that it can phosphorylate its substrates and that tetraspanin-containing microdomains can act as signaling hotspots in the plasma membrane. 2017",
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Zuidscherwoude, M, Dunlock, VME, Van Den Bogaart, G, Van Deventer, SJ, van der Schaaf, A, Van Oostrum, J, Goedhart, J, In't Hout, J, Hämmerling, GJ, Tanaka, S, Nadler, A, Schultz, C, Wright, MD, Adjobo-Hermans, MJW & Van Spriel, AB 2017, 'Tetraspanin microdomains control localized protein kinase C signaling in B cells', Science Signaling, vol. 10, no. 478, aag2755. https://doi.org/10.1126/scisignal.aag2755

Tetraspanin microdomains control localized protein kinase C signaling in B cells. / Zuidscherwoude, Malou; Dunlock, Vera Marie E.; Van Den Bogaart, Geert; Van Deventer, Sjoerd J.; van der Schaaf, Alie; Van Oostrum, Jenny; Goedhart, Joachim; In't Hout, Joanna; Hämmerling, Günter J.; Tanaka, Satoshi; Nadler, André; Schultz, Carsten; Wright, Mark D.; Adjobo-Hermans, Merel J.W.; Van Spriel, Annemiek B.

In: Science Signaling, Vol. 10, No. 478, aag2755, 09.05.2017.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Tetraspanin microdomains control localized protein kinase C signaling in B cells

AU - Zuidscherwoude, Malou

AU - Dunlock, Vera Marie E.

AU - Van Den Bogaart, Geert

AU - Van Deventer, Sjoerd J.

AU - van der Schaaf, Alie

AU - Van Oostrum, Jenny

AU - Goedhart, Joachim

AU - In't Hout, Joanna

AU - Hämmerling, Günter J.

AU - Tanaka, Satoshi

AU - Nadler, André

AU - Schultz, Carsten

AU - Wright, Mark D.

AU - Adjobo-Hermans, Merel J.W.

AU - Van Spriel, Annemiek B.

PY - 2017/5/9

Y1 - 2017/5/9

N2 - Activation of B cells by the binding of antigens to the B cell receptor (BCR) requires the protein kinase C (PKC) family member PKCβ. Because PKCs must translocate to the plasma membrane to become activated, we investigated the mechanisms regulating their spatial distribution in mouse and human B cells. Through live-cell imaging, we showed that BCR-stimulated production of the second messenger diacylglycerol (DAG) resulted in the translocation of PKCβ from the cytosol to plasma membrane regions containing the tetraspanin protein CD53. CD53 was specifically enriched at sites of BCR signaling, suggesting that BCR-dependent PKC signaling was initiated at these tetraspanin microdomains. Fluorescence lifetime imaging microscopy studies confirmed the molecular recruitment of PKC to CD53-containing microdomains, which required the amino terminus of CD53. Furthermore, we showed that Cd53-deficient B cells were defective in the phosphorylation of PKC substrates. Consistent with this finding, PKC recruitment to the plasma membrane was impaired in both mouse and human CD53-deficient B cells compared to that in their wild-type counterparts. These data suggest that CD53 promotes BCR-dependent PKC signaling by recruiting PKC to the plasma membrane so that it can phosphorylate its substrates and that tetraspanin-containing microdomains can act as signaling hotspots in the plasma membrane. 2017

AB - Activation of B cells by the binding of antigens to the B cell receptor (BCR) requires the protein kinase C (PKC) family member PKCβ. Because PKCs must translocate to the plasma membrane to become activated, we investigated the mechanisms regulating their spatial distribution in mouse and human B cells. Through live-cell imaging, we showed that BCR-stimulated production of the second messenger diacylglycerol (DAG) resulted in the translocation of PKCβ from the cytosol to plasma membrane regions containing the tetraspanin protein CD53. CD53 was specifically enriched at sites of BCR signaling, suggesting that BCR-dependent PKC signaling was initiated at these tetraspanin microdomains. Fluorescence lifetime imaging microscopy studies confirmed the molecular recruitment of PKC to CD53-containing microdomains, which required the amino terminus of CD53. Furthermore, we showed that Cd53-deficient B cells were defective in the phosphorylation of PKC substrates. Consistent with this finding, PKC recruitment to the plasma membrane was impaired in both mouse and human CD53-deficient B cells compared to that in their wild-type counterparts. These data suggest that CD53 promotes BCR-dependent PKC signaling by recruiting PKC to the plasma membrane so that it can phosphorylate its substrates and that tetraspanin-containing microdomains can act as signaling hotspots in the plasma membrane. 2017

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U2 - 10.1126/scisignal.aag2755

DO - 10.1126/scisignal.aag2755

M3 - Article

VL - 10

JO - Science Signaling

JF - Science Signaling

SN - 1945-0877

IS - 478

M1 - aag2755

ER -

Zuidscherwoude M, Dunlock VME, Van Den Bogaart G, Van Deventer SJ, van der Schaaf A, Van Oostrum J et al. Tetraspanin microdomains control localized protein kinase C signaling in B cells. Science Signaling. 2017 May 9;10(478). aag2755. https://doi.org/10.1126/scisignal.aag2755