Tetraspanin CD82 restrains phagocyte migration but supports macrophage activation

Erin N.S. McGowan, Osanna Wong, Eleanor Jones, Julie Nguyen, Janet Wee, Maria C. Demaria, Devy Deliyanti, Chad J. Johnson, Michael J. Hickey, Malcolm J. McConville, Jennifer L. Wilkinson-Berka, Mark D. Wright, Katrina J. Binger

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)


Phagocytes migrate into tissues to combat infection and maintain tissue homeostasis. As dysregulated phagocyte migration and function can lead to inflammation or susceptibility to infection, identifying molecules that control these processes is critical. Here, we show that the tetraspanin CD82 restrains the migration of neutrophils and macrophages into tissues. Cd82−/− phagocytes exhibited excessive migration during in vivo models of peritoneal inflammation, superfusion of CXCL1, retinopathy of prematurity, and infection with the protozoan parasite L. mexicana. However, with the latter, while Cd82−/− macrophages infiltrated infection sites at higher proportions, cutaneous L. mexicana lesions were larger and persisted, indicating a failure to control infection. Analyses of in vitro bone-marrow-derived macrophages showed CD82 deficiency altered cellular morphology, and impaired gene expression and metabolism in response to anti-inflammatory activation. Altogether, this work reveals an important role for CD82 in restraining phagocyte infiltration and mediating their differentiation in response to stimulatory cues.

Original languageEnglish
Article number104520
Number of pages21
Issue number7
Publication statusPublished - 15 Jul 2022


  • Biological sciences
  • Immune system
  • Immunology

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