Tetraspanin CD53 modulates lymphocyte trafficking but not systemic autoimmunity in Lyn-deficient mice

Louisa Yeung, Timothy A. Gottschalk, Pam Hall, Evelyn Tsantikos, Rebecca H. Gallagher, A. Richard Kitching, Margaret L. Hibbs, Mark D. Wright, Michael J. Hickey

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)


The leukocyte-restricted tetraspanin CD53 has been shown to promote lymphocyte homing to lymph nodes (LNs) and myeloid cell recruitment to acutely inflamed peripheral organs, and accelerate the onset of immune-mediated disease. However, its contribution in the setting of chronic systemic autoimmunity has not been investigated. We made use of the Lyn−/− autoimmune model, generating Cd53−/−Lyn−/− mice, and compared trafficking of immune cells into secondary lymphoid organs and systemic autoimmune disease development with mice lacking either gene alone. Consistent with previous observations, absence of CD53 led to reduced LN cellularity via reductions in both B and T cells, a phenotype also observed in Cd53−/−Lyn−/− mice. In some settings, Cd53−/−Lyn−/− lymphocytes showed greater loss of surface L-selectin and CD69 upregulation above that imparted by Lyn deficiency alone, indicating that absence of these two proteins can mediate additive effects in the immune system. Conversely, prototypical effects of Lyn deficiency including splenomegaly, plasma cell expansion, elevated serum immunoglobulin M and anti-nuclear antibodies were unaffected by CD53 deficiency. Furthermore, while Lyn−/− mice developed glomerular injury and showed elevated glomerular neutrophil retention above than that in wild-type mice, absence of CD53 in Lyn−/− mice did not alter these responses. Together, these findings demonstrate that while tetraspanin CD53 promotes lymphocyte trafficking into LNs independent of Lyn, it does not make an important contribution to development of autoimmunity, plasma cell dysfunction or glomerular injury in the Lyn−/− model of systemic autoimmunity.

Original languageEnglish
Pages (from-to)1053-1066
Number of pages14
JournalImmunology and Cell Biology
Issue number10
Publication statusPublished - Nov 2021


  • cell migration
  • inflammation
  • lupus nephritis
  • lymphocyte activation
  • mechanisms of disease
  • systemic lupus erythematosus

Cite this