Tetraspanin CD37 contributes to the initiation of cellular immunity by promoting dendritic cell migration

Kate Gartlan, Janet Lye-Keng Wee, Maria Demaria, Roza Nastovska, Tsz Man (Fiona) Chang, Eleanor Jones, Vasso Apostolopoulos, Geoffrey A Pietersz, Michael John Hickey, Annemiek B van Spriel, Mark Dexter Wright

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Previous studies on the role of the tetraspanin CD37 in cellular immunity appear contradictory. In vitro approaches indicate a negative regulatory role, whereas in vivo studies suggest that CD37 is necessary for optimal cellular responses. To resolve this discrepancy, we studied the adaptive cellular immune responses of CD37-/- mice to intradermal challenge with either tumors or model antigens and found that CD37 is essential for optimal cell-mediated immunity. We provide evidence that an increased susceptibility to tumors observed in CD37-/- mice coincides with a striking failure to induce antigen-specific IFN-gamma-secreting T cells. We also show that CD37 ablation impairs several aspects of DC function including: in vivo migration from skin to draining lymph nodes; chemo-tactic migration; integrin-mediated adhesion under flow; the ability to spread and form actin protrusions and in vivo priming of adoptively transferred naive T cells. In addition, multiphoton microscopy-based assessment of dermal DC migration demonstrated a reduced rate of migration and increased randomness of DC migration in CD37-/- mice. Together, these studies are consistent with a model in which the cellular defect that underlies poor cellular immune induction in CD37-/- mice is impaired DC migration.
Original languageEnglish
Pages (from-to)1208 - 1219
Number of pages12
JournalEuropean Journal of Immunology
Volume43
Issue number5
DOIs
Publication statusPublished - 2013

Cite this

Gartlan, Kate ; Wee, Janet Lye-Keng ; Demaria, Maria ; Nastovska, Roza ; Chang, Tsz Man (Fiona) ; Jones, Eleanor ; Apostolopoulos, Vasso ; Pietersz, Geoffrey A ; Hickey, Michael John ; van Spriel, Annemiek B ; Wright, Mark Dexter. / Tetraspanin CD37 contributes to the initiation of cellular immunity by promoting dendritic cell migration. In: European Journal of Immunology. 2013 ; Vol. 43, No. 5. pp. 1208 - 1219.
@article{cf97a53d8a9843b3956fb254f245ce2c,
title = "Tetraspanin CD37 contributes to the initiation of cellular immunity by promoting dendritic cell migration",
abstract = "Previous studies on the role of the tetraspanin CD37 in cellular immunity appear contradictory. In vitro approaches indicate a negative regulatory role, whereas in vivo studies suggest that CD37 is necessary for optimal cellular responses. To resolve this discrepancy, we studied the adaptive cellular immune responses of CD37-/- mice to intradermal challenge with either tumors or model antigens and found that CD37 is essential for optimal cell-mediated immunity. We provide evidence that an increased susceptibility to tumors observed in CD37-/- mice coincides with a striking failure to induce antigen-specific IFN-gamma-secreting T cells. We also show that CD37 ablation impairs several aspects of DC function including: in vivo migration from skin to draining lymph nodes; chemo-tactic migration; integrin-mediated adhesion under flow; the ability to spread and form actin protrusions and in vivo priming of adoptively transferred naive T cells. In addition, multiphoton microscopy-based assessment of dermal DC migration demonstrated a reduced rate of migration and increased randomness of DC migration in CD37-/- mice. Together, these studies are consistent with a model in which the cellular defect that underlies poor cellular immune induction in CD37-/- mice is impaired DC migration.",
author = "Kate Gartlan and Wee, {Janet Lye-Keng} and Maria Demaria and Roza Nastovska and Chang, {Tsz Man (Fiona)} and Eleanor Jones and Vasso Apostolopoulos and Pietersz, {Geoffrey A} and Hickey, {Michael John} and {van Spriel}, {Annemiek B} and Wright, {Mark Dexter}",
year = "2013",
doi = "10.1002/eji.201242730",
language = "English",
volume = "43",
pages = "1208 -- 1219",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag GmbH & Co. KGaA",
number = "5",

}

Gartlan, K, Wee, JL-K, Demaria, M, Nastovska, R, Chang, TMF, Jones, E, Apostolopoulos, V, Pietersz, GA, Hickey, MJ, van Spriel, AB & Wright, MD 2013, 'Tetraspanin CD37 contributes to the initiation of cellular immunity by promoting dendritic cell migration' European Journal of Immunology, vol. 43, no. 5, pp. 1208 - 1219. https://doi.org/10.1002/eji.201242730

Tetraspanin CD37 contributes to the initiation of cellular immunity by promoting dendritic cell migration. / Gartlan, Kate; Wee, Janet Lye-Keng; Demaria, Maria; Nastovska, Roza; Chang, Tsz Man (Fiona); Jones, Eleanor; Apostolopoulos, Vasso; Pietersz, Geoffrey A; Hickey, Michael John; van Spriel, Annemiek B; Wright, Mark Dexter.

In: European Journal of Immunology, Vol. 43, No. 5, 2013, p. 1208 - 1219.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Tetraspanin CD37 contributes to the initiation of cellular immunity by promoting dendritic cell migration

AU - Gartlan, Kate

AU - Wee, Janet Lye-Keng

AU - Demaria, Maria

AU - Nastovska, Roza

AU - Chang, Tsz Man (Fiona)

AU - Jones, Eleanor

AU - Apostolopoulos, Vasso

AU - Pietersz, Geoffrey A

AU - Hickey, Michael John

AU - van Spriel, Annemiek B

AU - Wright, Mark Dexter

PY - 2013

Y1 - 2013

N2 - Previous studies on the role of the tetraspanin CD37 in cellular immunity appear contradictory. In vitro approaches indicate a negative regulatory role, whereas in vivo studies suggest that CD37 is necessary for optimal cellular responses. To resolve this discrepancy, we studied the adaptive cellular immune responses of CD37-/- mice to intradermal challenge with either tumors or model antigens and found that CD37 is essential for optimal cell-mediated immunity. We provide evidence that an increased susceptibility to tumors observed in CD37-/- mice coincides with a striking failure to induce antigen-specific IFN-gamma-secreting T cells. We also show that CD37 ablation impairs several aspects of DC function including: in vivo migration from skin to draining lymph nodes; chemo-tactic migration; integrin-mediated adhesion under flow; the ability to spread and form actin protrusions and in vivo priming of adoptively transferred naive T cells. In addition, multiphoton microscopy-based assessment of dermal DC migration demonstrated a reduced rate of migration and increased randomness of DC migration in CD37-/- mice. Together, these studies are consistent with a model in which the cellular defect that underlies poor cellular immune induction in CD37-/- mice is impaired DC migration.

AB - Previous studies on the role of the tetraspanin CD37 in cellular immunity appear contradictory. In vitro approaches indicate a negative regulatory role, whereas in vivo studies suggest that CD37 is necessary for optimal cellular responses. To resolve this discrepancy, we studied the adaptive cellular immune responses of CD37-/- mice to intradermal challenge with either tumors or model antigens and found that CD37 is essential for optimal cell-mediated immunity. We provide evidence that an increased susceptibility to tumors observed in CD37-/- mice coincides with a striking failure to induce antigen-specific IFN-gamma-secreting T cells. We also show that CD37 ablation impairs several aspects of DC function including: in vivo migration from skin to draining lymph nodes; chemo-tactic migration; integrin-mediated adhesion under flow; the ability to spread and form actin protrusions and in vivo priming of adoptively transferred naive T cells. In addition, multiphoton microscopy-based assessment of dermal DC migration demonstrated a reduced rate of migration and increased randomness of DC migration in CD37-/- mice. Together, these studies are consistent with a model in which the cellular defect that underlies poor cellular immune induction in CD37-/- mice is impaired DC migration.

UR - http://www.ncbi.nlm.nih.gov/pubmed/23420539

U2 - 10.1002/eji.201242730

DO - 10.1002/eji.201242730

M3 - Article

VL - 43

SP - 1208

EP - 1219

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 5

ER -