TY - JOUR
T1 - Tethered bilayer lipid membranes based on monolayers of thiolipids mixed with a complementary dilution molecule. 1. Incorporation of channel peptides
AU - He, Lizhong
AU - Robertson, Joseph W.F.
AU - Li, Jing
AU - Kärcher, Iris
AU - Schiller, Stefan M.
AU - Knoll, Wolfgang
AU - Naumann, Renate
PY - 2005/12/6
Y1 - 2005/12/6
N2 - Tethered bilayer lipid membranes (tBLMs) are described based on the self-assembly of a monolayer on template stripped gold of an archea analogue thiolipid, 2,3-di-o-phytanyl-sn-glycerol-1-tetraethylene glycol-D, L-aα-lipoic acid ester lipid (DPTL), and a newly designed dilution molecule, tetraethylene glycol-D,L-α-lipoic acid ester (TEGL). The tBLM is completed by fusion of liposomes made from a mixture of diphytanoylphosphatidyl choline (DPhyPC), cholesterol, and 1,2-diphytanoyl-sn-glycero-3-phosphate (DPhyPG) in a molar ratio of 6:3:1. Melittin and gramicidin are incorporated into these tBLMs as shown by surface plasmon resonance (SPR) and electrochemical impedance spectroscopy (EIS) studies. Ionic conductivity at 0 V vs Ag|AgCl, 3 M KCl, measured by EIS measurements are comparable to the results obtained by other research groups. Admittance plots as a function of potential are discussed on a qualitative basis in terms of the kinetics of ion transport through the channels.
AB - Tethered bilayer lipid membranes (tBLMs) are described based on the self-assembly of a monolayer on template stripped gold of an archea analogue thiolipid, 2,3-di-o-phytanyl-sn-glycerol-1-tetraethylene glycol-D, L-aα-lipoic acid ester lipid (DPTL), and a newly designed dilution molecule, tetraethylene glycol-D,L-α-lipoic acid ester (TEGL). The tBLM is completed by fusion of liposomes made from a mixture of diphytanoylphosphatidyl choline (DPhyPC), cholesterol, and 1,2-diphytanoyl-sn-glycero-3-phosphate (DPhyPG) in a molar ratio of 6:3:1. Melittin and gramicidin are incorporated into these tBLMs as shown by surface plasmon resonance (SPR) and electrochemical impedance spectroscopy (EIS) studies. Ionic conductivity at 0 V vs Ag|AgCl, 3 M KCl, measured by EIS measurements are comparable to the results obtained by other research groups. Admittance plots as a function of potential are discussed on a qualitative basis in terms of the kinetics of ion transport through the channels.
UR - http://www.scopus.com/inward/record.url?scp=29444456640&partnerID=8YFLogxK
U2 - 10.1021/la051771p
DO - 10.1021/la051771p
M3 - Article
C2 - 16316098
AN - SCOPUS:29444456640
SN - 0743-7463
VL - 21
SP - 11666
EP - 11672
JO - Langmuir
JF - Langmuir
IS - 25
ER -