Abstract
A new cardiac stimulatory protein, tenebrosin-A, has been isolated from the Australian sea anemone Actinia tenebrosa by gel filtration and cation-exchange chromatography, followed by cation-exchange HPLC. Its purity is established by analytical reversed-phase HPLC and N-terminal sequence analysis. According to SDS-PAGE, its apparent Mr is 20,000 daltons. Amino acid analysis indicates that it contains 186 residues, and is devoid of cysteine or cystine. Tenebrosin-A exerts a strong positive inotropic effect on isolated guinea pig atria at a concentration of 1.4 nM, with little chronotropic activity.
| Original language | English |
|---|---|
| Pages (from-to) | 711-718 |
| Number of pages | 8 |
| Journal | Biochemistry International |
| Volume | 15 |
| Issue number | 4 |
| Publication status | Published - 1987 |
| Externally published | Yes |