Tenebrosin-A, a new cardiostimulant protein from the Australian sea anemone Actinia tenebrosa

M. Thomson, R. L. Moritz, R. J. Simpson, R. S. Norton

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Abstract

A new cardiac stimulatory protein, tenebrosin-A, has been isolated from the Australian sea anemone Actinia tenebrosa by gel filtration and cation-exchange chromatography, followed by cation-exchange HPLC. Its purity is established by analytical reversed-phase HPLC and N-terminal sequence analysis. According to SDS-PAGE, its apparent Mr is 20,000 daltons. Amino acid analysis indicates that it contains 186 residues, and is devoid of cysteine or cystine. Tenebrosin-A exerts a strong positive inotropic effect on isolated guinea pig atria at a concentration of 1.4 nM, with little chronotropic activity.

Original languageEnglish
Pages (from-to)711-718
Number of pages8
JournalBiochemistry International
Volume15
Issue number4
Publication statusPublished - 1987
Externally publishedYes

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