Tenascin-R plays a role in neuroprotection via its distinct domains that coordinate to modulate the microglia function

Hong Liao, Wen-yu Bu, Ting-Hua Wang, Sohail Ahmed, Zhi-Cheng Xiao

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57 Citations (Scopus)

Abstract

Microglia are one of the main cell types activated by brain injury. In the present study, we have investigated how domains of the extracellular matrix molecule tenascin-R (TN-R) modulate microglia function. We found that epidermal growth factor-like repeats inhibited adhesion and migration of microglia via a protein kinase A-dependent mechanism. In contrast, fibronectin 6-8 repeats promoted adhesion and migration of the primary microglia via a protein kinase C-dependent mechanism. Both domains of TN-R induced an up-regulation in the secretion of cytokines, such as chemokine-induced cytokine 3 and tumor neurosis factor alpha. Interestingly, epidermal growth factor-like repeats and fibronectin 6-8 induced a dramatic up-regulation in the secretion of brain-derived neurotrophic factor/transforming growth factor-beta and nerve growth factor/transforming growth factor-beta, respectively, and conditioned medium from activated microglia was able to promote neurite outgrowth of N1E-115 cells and primary cortical neurons. These results suggest that TN-R plays a role in neuroprotection through distinct domains coordinating to modulate microglia function.
Original languageEnglish
Pages (from-to)8316 - 8323
Number of pages8
JournalThe Journal of Biological Chemistry
Volume280
Issue number9
DOIs
Publication statusPublished - 2005
Externally publishedYes

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