Temporally-controlled site-specific recombination in zebrafish

Stefan Hans, Jan Victor Kaslin, Dorian Freudenreich, Michael Brand

Research output: Contribution to journalArticleResearchpeer-review

140 Citations (Scopus)

Abstract

Conventional use of the site-specific recombinase Cre is a powerful technology in mouse, but almost absent in other vertebrate model organisms. In zebrafish, Cre-mediated recombination efficiency was previously very low. Here we show that using transposon-mediated transgenesis, Cre is in fact highly efficient in this organism. Furthermore, temporal control of recombination can be achieved by using the ligand-inducible CreER(T2). Site-specific recombination only occurs upon administration of the drug tamoxifen (TAM) or its active metabolite, 4-hydroxy-tamoxifen (4-OHT). Cre-mediated recombination is detectable already 4 or 2 hours after administration of TAM or 4-OHT, demonstrating fast recombination kinetics. In addition, low doses of TAM allow mosaic labeling of single cells. Combined, our results show that conditional Cre/lox will be a valuable tool for both, embryonic and adult zebrafish studies. Furthermore, single copy insertion transgenesis of Cre/lox constructs suggest a strategy suitable also for other organisms.
Original languageEnglish
Article numbere4640
Number of pages7
JournalPLoS ONE
Volume4
Issue number2
DOIs
Publication statusPublished - 2009
Externally publishedYes

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